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Stability and mechanical evaluation of bovine pericardium cross-linked with polyurethane prepolymer in aqueous medium

2013; Elsevier BV; Volume: 33; Issue: 4 Linguagem: Inglês

10.1016/j.msec.2013.02.001

1873-0191

Birzabith Mendoza‐Novelo, Diego I. Alvarado-Castro, José L. Mata‐Mata, Juan Valerio Cauich‐Rodríguez, Arturo Vega‐González, E. Jorge‐Herrero, Francisco J. Rojo, Gustavo V. Guinea,

biodegradable polymer synthesis and properties

The present study investigates the potential use of non-catalyzed water-soluble blocked polyurethane prepolymer (PUP) as a bifunctional cross-linker for collagenous scaffolds. The effect of concentration (5, 10, 15 and 20%), time (4, 6, 12 and 24 h), medium volume (50, 100, 200 and 300%) and pH (7.4, 8.2, 9 and 10) over stability, microstructure and tensile mechanical behavior of acellular pericardial matrix was studied. The cross-linking index increased up to 81% while the denaturation temperature increased up to 12 °C after PUP crosslinking. PUP-treated scaffold resisted the collagenase degradation (0.167 ± 0.14 mmol/g of liberated amine groups vs. 598 ± 60 mmol/g for non-cross-linked matrix). The collagen fiber network was coated with PUP while viscoelastic properties were altered after cross-linking. The treatment of the pericardial scaffold with PUP allows (i) different densities of cross-linking depending of the process parameters and (ii) tensile properties similar to glutaraldehyde method.