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Confocal laser scanning microscopy and three-dimensional reconstruction of cell clusters in serous fluids

1997; Wiley; Volume: 17; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-0339(199710)17

8755-1039

Claire W. Michael, Judy King, Raymond B. Hester,

Cellular Mechanics and Interactions

Diagnostic CytopathologyVolume 17, Issue 4 p. 272-279 Focus on Technology Confocal laser scanning microscopy and three-dimensional reconstruction of cell clusters in serous fluids Claire W. Michael M.D., Corresponding Author Claire W. Michael M.D. Department of Pathology, University of Michigan, Ann Arbor, MichiganDepartment of Pathology, University of Michigan, 1500 E. Medical Center Drive, Room 2G332/Box 0054, Ann Arbor, MI 48109-0054Search for more papers by this authorJudy A.C. King M.D., Ph.D., Judy A.C. King M.D., Ph.D. Department of Pathology, University of South Alabama, Mobile, AlabamaSearch for more papers by this authorRaymond B. Hester Ph.D., Raymond B. Hester Ph.D. Department of Microbiology, University of South Alabama, Mobile, AlabamaSearch for more papers by this author Claire W. Michael M.D., Corresponding Author Claire W. Michael M.D. Department of Pathology, University of Michigan, Ann Arbor, MichiganDepartment of Pathology, University of Michigan, 1500 E. Medical Center Drive, Room 2G332/Box 0054, Ann Arbor, MI 48109-0054Search for more papers by this authorJudy A.C. King M.D., Ph.D., Judy A.C. King M.D., Ph.D. Department of Pathology, University of South Alabama, Mobile, AlabamaSearch for more papers by this authorRaymond B. Hester Ph.D., Raymond B. Hester Ph.D. Department of Microbiology, University of South Alabama, Mobile, AlabamaSearch for more papers by this author First published: 07 December 1998 https://doi.org/10.1002/(SICI)1097-0339(199710)17:4 3.0.CO;2-ECitations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Thick cell clusters are a common finding in reactive and malignant effusions. In order to arrive at a diagnosis, clusters are evaluated for certain cytomorphologic features including size, shape, smooth vs. scalloped borders, and three-dimensional (3-D) configuration. By conventional microscopy, the image of these clusters is often blurred due to limitations in resolution. Consequently, the exact internal structure and cellular arrangement within these clusters cannot be adequately determined. Utilizing confocal laser scanning microscopy (CLSM), we examined serous fluids from a variety of conditions. Cases included mesothelioma, adenocarcinoma, and papillary adenocarcinoma. Smears were stained with 0.01% ethidium bromide and 1% eosin Y, followed by analysis with an ACAS 570™ image analyzer (Meridian Instruments, Inc. Okemos, MI). Serial confocal fluorescence images were acquired, which allowed 3-D reconstruction of the clusters. Mesothelioma clusters (excluding those with obvious central collagen cores by light microscopy) appeared to be formed of the following configurations: 1) randomly coiled cords of cells, 2) small papillae encompassing central cores, and 3) tissue fragments with pseudoacinar formation. In contrast, adenocarcinomas had a more orderly pattern, with tightly cohesive cells and true acinar formation. Diagn. Cytopathol. 1997;17: 272–279. © 1997 Wiley-Liss, Inc. References 1 Shorten DM. Confocal scanning optical microscopy and its applications for biological specimens. J Cell Sci 1989; 94: 175–206. 2 Sasano H, Date F, Itakura Y, Goukon Y, Nishihira T, Nagura H. Confocal laser scanning microscopy in cytopathology. Mod Pathol 1993; 6: 625–629. 3 Tekola P, Zhu Q, Baak JPA. 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Citing Literature Volume17, Issue4October 1997Pages 272-279 ReferencesRelatedInformation