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A new spectrally distinct component in preparations of chlorophyll c from the micro-alga Emiliania huxleyi (Prymnesiophycease)

1987; Elsevier BV; Volume: 894; Issue: 2 Linguagem: Inglês

10.1016/0005-2728(87)90188-5

1879-2650

S. W. Jeffrey, Simon W. Wright,

Biocrusts and Microbial Ecology

A new chlorophyll c pigment designated chlorophyll c3 has been isolated from the coccolithophorid Emiliania huxleyi (Prymnesiophyceae) using reverse-phase high-performance thin-layer chromatography (HPTLC). Its spectral properties were compared with chlorophylls c1 and c2 from standard sources. Visible absorption maxima of the new pigment in diethyl ether were at 451, 585 and 625 nm with band ratios of 30.77, 3.79 and 1.00, respectively. Chlorophyll c2 was present in approximately equal proportions to chlorophyll c3, with maxima in diethyl ether at 447, 579 and 628 nm and band ratios of 12.26, 1.17 and 1.00, respectively. No chlorophyll c1 was detected. The visible absorption spectra of the magnesium-free derivatives of both chlorophylls c2 and c3 from E. huxleyi in acetone were also recorded. The new chlorophyll c3 pigment was chromatographically and spectrally distinct from a similar pigment, magnesium 2,4-divinylpheoporphyrin a5 monomethyl ester, present in prasinophyte algae, with which it could have been confused.