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First Report of Teratosphaeria pseudoeucalypti on Eucalyptus Hybrids in Argentina

2014; American Phytopathological Society; Volume: 99; Issue: 4 Linguagem: Inglês

10.1094/pdis-10-14-1087-pdn

1943-7692

Sergio Ramos, Carlos A. Pérez,

Forest Insect Ecology and Management

HomePlant DiseaseVol. 99, No. 4First Report of Teratosphaeria pseudoeucalypti on Eucalyptus Hybrids in Argentina PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Teratosphaeria pseudoeucalypti on Eucalyptus Hybrids in ArgentinaS. O. Ramos and C. A. PerezS. O. RamosSearch for more papers by this author and C. A. PerezSearch for more papers by this authorAffiliationsAuthors and Affiliations S. O. Ramos , Protección Forestal Estación Experimental Agropecuaria Concordia, Instituto Nacional de Tecnología Agropecuaria. CC N° 34 (E3200AQK), Concordia, Entre Ríos, Argentina C. A. Perez , Departamento de Protección Vegetal, Universidad de la República, Ruta 3, km 363, Paysandú, Uruguay. Published Online:1 Apr 2015https://doi.org/10.1094/PDIS-10-14-1087-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Mycosphaerella leaf disease (MLD), also called Teratosphaeria leaf disease (TLD), is among the most significant foliar diseases on eucalyptus, Teratosphaeria nubilosa and T. destructans being the most devastating species affecting eucalyptus plantations worldwide. Recently, T. pseudoeucalypti severely affecting Eucalyptus hybrids was described in Australia (3). In Argentina, during a survey of clonal E. grandis × E. camaldulensis hybrid trials, symptoms resembling those described for T. pseudoeucalypti were observed in Entre Ríos province in September 2013. The symptoms observed in young leaves were large yellow chlorotic spots and deformed leaves, whereas in older leaves they were individual pale brown necrotic spots. Symptomatic leaves were collected and put in moist chambers to induce fructification and sporulation. A total of 30 conidia were measured in length and width. Pure cultures were obtained according to the method of Andjic et al. (1). DNA extraction from three pure isolates (ERI5, ERI6, and ERI12) was performed using a CTAB protocol (2). Three genomic regions, ITS, EF-1α, and β-tubulin, were amplified by using specific primers ITS-3 and ITS-4, EF1-728F and EF1-986R, and Bt2a and Bt2b, respectively. Sequences obtained from isolates ERI5, ERI6, and ERI12 were deposited in GenBank (Accession Nos. KP006702, KP006705, and KP006708 for ITS; KP006701, KP006704, and KP006707, for EF-1α; and KP006700, KP006703, and KP006706 for β-tubulin, respectively). Phylogenetic analysis of combined ITS, Ef-1α, and β-tubulin datasets of closely related species from a BLAST search was performed by using PAUP Version 4.0b10 for neighbor-joining and maximum parsimony. The aligned dataset was deposited in TreeBASE (ID 16562). The conidia were pale brown and zero- to one-septate, and the conidial size was (31) 37.5 (43.7) μm long and (2) 2.6 (3.2) μm wide, which is a little longer than those reported for T. pseudoeucalypti. The cultural characteristics of the three isolates on malt extract agar (MEA) were similar to those described for T. pseudoeucalypti. Growth was slow, reaching 10 mm after 30 days on MEA at 25°C. White mycelia were seen on the upper side and dark color underneath. The phylogenetic analysis combining the three genomic regions grouped these isolates with T. pseudoeucalypti in a well-supported clade (>90% bootstrap support). To our knowledge, this is the first report of T. pseudoeucalypti in Argentina and represents the second report of this pathogen outside of Australia, after Uruguay (4). This disease constitutes a serious threat for development of cold-tolerant Eucalyptus hybrids in Argentina. Further investigation is required to determine the extent of this disease in Argentinean forest plantations. Fortunately, differences in susceptibility have been observed in Eucalyptus hybrids from local sources, which may enable the selection of resistant genotypes.References:(1) Andjic, V., et al. 2007. Australas. Plant Pathol. 36:478. https://doi.org/10.1071/AP07054 Crossref, ISI, Google Scholar(2) Andjic, V., et al. 2007. FEMS Microbiol. Lett. 268:22. https://doi.org/10.1111/j.1574-6968.2007.00637.x Crossref, ISI, Google Scholar(3) Andjic, V., et al. 2010. Plant Pathol. 59:900. https://doi.org/10.1111/j.1365-3059.2010.02308.x Crossref, ISI, Google Scholar(4) Soria, S., et al. 2014. Australas. Plant Dis. Notes 9:146. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 4 April 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 Apr 2015Published: 1 Apr 2015First Look: 11 Dec 2014Accepted: 21 Nov 2014 Pages: 554-554 Information© 2015 The American Phytopathological SocietyCited byTeratosphaeria pseudoeucalypti (Teratosphaeria leaf disease)CABI Compendium, Vol. CABI CompendiumGenetic diversity of Teratosphaeria pseudoeucalypti in Eucalyptus plantations in Australia and Uruguay16 June 2021 | Australasian Plant Pathology, Vol. 50, No. 6IMA Genome - F1324 September 2020 | IMA Fungus, Vol. 11, No. 1Diseases of Eucalyptus Plantations in Uruguay: Current State and Management Alternatives27 February 202023 years of research on Teratosphaeria leaf blight of EucalyptusForest Ecology and Management, Vol. 443Chemical Control of Mycosphaerella Leaf Disease on Eucalyptus dunnii in Southern Brazil21 May 2018 | Floresta e Ambiente, Vol. 25, No. 2Pathogens on the Move: A 100-Year Global Experiment with Planted Eucalypts7 December 2016 | BioScience, Vol. 67, No. 1New cryptic species of Teratosphaeria on Eucalyptus in Australia20 October 2016 | IMA Fungus, Vol. 7, No. 2