Regulation of Macrophage Colony-Stimulating Factor (M-CSF) Production in Cultured Human Synovial Fibroblasts
1993; Taylor & Francis; Volume: 9; Issue: 2 Linguagem: Inglês
10.3109/08977199309010831
ISSN1029-2292
AutoresOhn a. Hamilton, Enrico L Filonzi, Gina Ianches,
Tópico(s)Macrophage Migration Inhibitory Factor
ResumoAbstractAbstractObjective. To study the regulation of macrophage-colony stimulating factor (M-CSF) formation in vitro by human synovial fibroblast-like cells.Methods. Human synovial cell explant cultures were established using cells from non-rheumatoid donors. M-CSF antigen was measured by immunoassay, and messenger RNA (mRNA) levels were determined by Northern blot.Results. The cytokines, interleukin-1 (IL-1), tumor necrosis factora (TNFα), interferon-γ (IFN-γ) and IL-4, increased production of M-CSF above constitutive levels. The presence of the cyclooxygenase inhibitor, indomethacin, potentiated the action of IL-1 on M-CSF synthesis, suggesting that an endogenous cydooxygenase product(s) can down-regulate M-CSF formation. Changes in M-CSF mRNA levels paralleled those in protein levels. The glucocorticoid, dexamethasone, and the retinoid, all-trans retinoic acid, stimulated M-CSF formation. The control of M-CSF synthesis in the synovial fibroblasts differs from that for granulocyte macrophage-CSF (GM-CSF) and granulocyte-CSF (G-CSF).Conclusion. These results suggest that cytokine-stimulated synovial fibroblasts may be a source of M-CSF production in the joints of patients with inflammatory arthritis; as a result, monocyte/macrophages may be activated, leading to perpetuation of the inflammation and destructive events occurring in these lesions.Key Words: macrophage-colony stimulating factorsynovial cells
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