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Super-activated interferon-regulatory factors can enhance plasmid immunization

2003; Elsevier BV; Volume: 21; Issue: 13-14 Linguagem: Inglês

10.1016/s0264-410x(02)00694-1

1873-2518

Jonathan L. Bramson, Kelley Dayball, Jennifer R. Hall, James Millar, Mark J. Miller, Yong Wan, Rongtuan Lin, John Hiscott,

Immune Cell Function and Interaction

We have been investigating the adjuvant properties of two super-activated interferon-regulatory factors (IRFs), IRF-3(5D) and IRF7/3A, identified in our previous studies of structure–function relationships, for enhancing plasmid vaccines. Intramuscular injection of plasmid cocktails encoding IRF-3(5D) and IRF7/3A molecules elicited cytotoxic T cell responses in over 80% of mice following a single immunization compared to a 20% response-rate using a control cocktail. Most interestingly, greater than 60% of mice immunized with the super-activated IRFs developed antigen-specific antibodies compared to 0% of the mice in the control group. Finally, vaccines which incorporated the super-activated IRFs provided greater protection against challenge with a recombinant vaccinia virus. These results support further investigation of the potential of these agents as adjuvants for genetic immunization.