Portal de Periódicos da CAPES

Occurrence and genotypes of Giardia isolated from lambs in Spain

2009; Elsevier BV; Volume: 58; Issue: 3 Linguagem: Inglês

10.1016/j.parint.2009.06.005

1873-0329

María Teresa Gómez-Muñoz, Cristina Navarro, M.M. Garijo, María Auxiliadora Dea‐Ayuela, Salceda Fernández-Barredo, María Teresa Pérez‐Gracia, María Victoria Domínguez-Márquez, Rafael Borrás,

Parasitic Infections and Diagnostics

Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and β-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. β-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of β-giardin Assemblage E are reported in this study. In each farm, one to six different β-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain.