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Improvement in neutrophil and monocyte function during highly active antiretroviral treatment of HIV-1-infected patients

1999; Lippincott Williams & Wilkins; Volume: 13; Issue: 8 Linguagem: Inglês

10.1097/00002030-199905280-00003

1473-5571

Claudio Maria Mastroianni, Miriam Lichtner, Fabio Mengoni, Claudia D‚Agostino, Gabriele Forcina, Gabriella d‚Ettorre, P Santopadre, Vincenzo Vullo,

Pneumocystis jirovecii pneumonia detection and treatment

Objective: To investigate the effect of highly active antiretroviral treatment (HAART) on neutrophil and monocyte function in patients with moderately advanced HIV-1 infection. Design: Eighteen HIV-1-infected patients with CD4 T cell counts below 350/ml, no concomitant active infection, and no previous use of protease inhibitors were treated with indinavir or ritonavir and two reverse-transcriptase inhibitors and were followed up for 9 months. Ten age- and sex-matched healthy subjects were included as controls. Methods: The functional activity of neutrophils and monocytes was measured by assessing chemotaxis towards a bacterial peptide, killing activity against Candida albicans, and oxidative burst as measured by chemiluminescence production. Results: Neutrophils and monocytes from the treatment group exhibited a significantly diminished baseline chemotactic and fungicidal activity compared with healthy controls (P<0.001). After starting HAART, there was a significant improvement in chemotaxis and fungicidal activity of phagocytic cells (P<0.001). Values of chemotaxis reached normal ranges in 13 out of 18 patients (72%) for neutrophils and eight out of 18 (44%) for monocytes, whereas phagocyte killing was rarely restored to normal values (3/18 cases for monocytes and 0/18 for neutrophils). The administration of HAART was also associated with significantly increased phagocyte chemiluminescence production in response to phorbol-12-myristate 13-acetate or opsonized C. albicans (P<0.01). Conclusion: The functional improvement of two critical components of innate antimicrobial immunity, such as neutrophils and monocytes, may contribute to the improved cell-mediated immune responses against opportunistic infections in HAART-treated patients.