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Antisense to NPY-Y1 demonstrates that Y1 receptors in the hypothalamus underlie NPY hypothermia and feeding in rats

1996; Royal Society; Volume: 263; Issue: 1372 Linguagem: Inglês

10.1098/rspb.1996.0130

1471-2954

Francisco J. Lopez-Valpuesta, Jonathan W. Nyce, T. A. Griffin-Biggs, John Ice, R.D. Myers,

Hypothalamic control of reproductive hormones

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Lopez-Valpuesta F. J. , Nyce J. W. , Griffin-Biggs T. A. , Ice J. C. and Myers R. D. 1996Antisense to NPY-Y1 demonstrates that Y1 receptors in the hypothalamus underlie NPY hypothermia and feeding in ratsProc. R. Soc. Lond. B.263881–886http://doi.org/10.1098/rspb.1996.0130SectionRestricted accessArticleAntisense to NPY-Y1 demonstrates that Y1 receptors in the hypothalamus underlie NPY hypothermia and feeding in rats F. J. Lopez-Valpuesta Google Scholar Find this author on PubMed Search for more papers by this author , J. W. Nyce Google Scholar Find this author on PubMed Search for more papers by this author , T. A. Griffin-Biggs Google Scholar Find this author on PubMed Search for more papers by this author , J. C. Ice Google Scholar Find this author on PubMed Search for more papers by this author and R. D. Myers Google Scholar Find this author on PubMed Search for more papers by this author F. J. Lopez-Valpuesta Google Scholar Find this author on PubMed , J. W. Nyce Google Scholar Find this author on PubMed , T. A. Griffin-Biggs Google Scholar Find this author on PubMed , J. C. Ice Google Scholar Find this author on PubMed and R. D. Myers Google Scholar Find this author on PubMed Published:22 July 1996https://doi.org/10.1098/rspb.1996.0130AbstractNeuropeptide Y (NPY) is a highly potent endogenous peptide which when injected into the medial hypothalamus causes spontaneous eating behaviour and an intense fall in body temperature (Tb). This study used antisense oligodeoxynucleotides (ODNs) to determine whether the Y1 subtype of NPY receptor could underlie these remarkable physiological responses. In the unrestrained rat, the ventromedial hypothalamus (VMH) which is highly reactive to NPY was injected with antisense for NPY (aNPY), Y1 receptors (aNPY-Y1) and mismatched controls (mNPY; mNPY-Y1). After cannulae were implanted bilaterally in the brain of 19 rats, 0.4 or 0.8 μg per 0.8 μl of the phosphorothioate synthesised ODNs were delivered to the VMH of the rats at 12 h intervals over 2 d. Only the lower dose of aNPY-Y1, but not aNPY, evoked an intense phasic rise in the Tb, following each micro-injection. Simultaneously, 0.4 μg per 0.8 μl of aNPY-Y1, but not aNPY, suppressed feeding behaviour after a sequence of micro-injections and on the following day. Body weights and locomotor activity of the rats likewise declined concomitantly with the hyperthermia and hypophagia caused by the Y1 receptor antisense. Neither of the control ODNs for NPY or Y1 receptors injected similarly in the VMH of the rats exerted any effects on these measures. These results clearly provide convincing evidence that in the VMH the Y1 subtype of NPY receptor mediates, in part, the neuronal mechanisms responsible for spontaneous feeding and hypothermia produced by native NPY when applied directly to this structure. The concurrent decline in body weight and activity caused by aNPY-Y1 could be caused by the episodes of hyperthermia.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. 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