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Adsorption of Alexa-Labeled Bt Toxin on Mica, Glass, and Hydrophobized Glass: Study by Normal Scanning Confocal Fluorescence

2010; American Chemical Society; Volume: 11; Issue: 6 Linguagem: Inglês

10.1021/bm100313n

1526-4602

Jean‐Marc Janot, Michel Boissière, Thierry Thami, E. Tronel-Peyroz, Nordine Helassa, Sylvie Noinville, Hervé Quiquampoix, Siobhán Staunton, Philippe Déjardin,

Protein purification and stability

We studied the kinetics of adsorption of alexa-labeled Bt toxin Cry1Aa, in monomer and oligomer states, on muscovite mica, acid-treated hydrophilic glass, and hydrophobized glass, in the configuration of laminar flow of solution in a slit. Normal confocal fluorescence through the liquid volume allows the visualization of the concentration in solution over the time of adsorption, in addition to the signal due to the adsorbed molecules at the interface. The solution signal is used as calibration for estimation of interfacial concentration. We found low adsorption of the monomer compared to oligomers on the three types of surface. The kinetic adsorption barrier for oligomers increases in the order hydrophobized glass, muscovite mica, acid-treated hydrophilic glass. This suggests enhanced immobilization in soil if toxin is under oligomer state.