Cypridina Bioluminescence: Light-Emitting Oxyluciferin-Luciferase Complex
1969; American Association for the Advancement of Science; Volume: 164; Issue: 3885 Linguagem: Inglês
10.1126/science.164.3885.1299
ISSN1095-9203
AutoresOsamu Shimomura, Frank H. Johnson, TAKASHI MASUGI,
Tópico(s)Photoreceptor and optogenetics research
ResumoFluorescence of Cypridina oxyluciferin is greatly enhanced when it is bound to luciferase; the spectrum is thereby shifted, so that it corresponds precisely to the emission spectrum characteristic of the bioluminescentoxidation of luciferin. Thus the oxyluciferin-luciferase complex is the lightemitter. The binding is equimolar, with dissociation constant K(D) equal to 3x 10(-7) mole per liter. The molecular weight of the luciferase, according to three different methods, is between 52,000 and 57,000; molecular activities of luciferase for the bioluminescence reaction and for the hydrolysis of oxy-luciferin are 1600 and 2 per minute, respectively.
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