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Automatic analysis for amperometrical recordings of exocytosis

2000; Elsevier BV; Volume: 103; Issue: 2 Linguagem: Inglês

10.1016/s0165-0270(00)00309-5

1872-678X

Fernando Segura, Miguel A. Brioso, José F. Gómez, José David Machado, Ricardo Borges,

Photoreceptor and optogenetics research

Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis.