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Quantitation of urinary hydroxypyridinium cross-links from collagen by high-performance liquid chromatography

1992; Taylor & Francis; Volume: 52; Issue: 7 Linguagem: Inglês

10.3109/00365519209115510

1502-7686

G. Kollerup, Gorm Thamsborg, H. Bhatia, O. H. Sørensen,

Bone health and osteoporosis research

Pyridinoline and deoxypyridinoline are intermolecular cross-links in mature collagen in bone and cartilage. The urinary excretion of the two compounds correlates well to bone turnover. A fast, sensitive, and accurate isocratic ion-pairing reverse-phase high-performance liquid chromatography method for measurement of pyridinoline and deoxypyridinoline in urine has been established. Intra- and inter-assay precision were 5–7% and 12–14%, respectively. Recovery for pyridinoline was 97.4% and for deoxypyridinoline 94.3%. The detection limit was 0.4 pmol. Pyridinoline:creatinine and deoxypyridinoline: creatinine ratios in healthy subjects, were 38.8 nmol:mmol and 13.0 nmol: mmol, respectively. Increased values of both cross-links were observed in children, in the age group 20–29 in both sexes, and in post-menopausal women.