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Toxic Shock Syndrome Toxin-1 Accelerated Collagen-induced Arthritis in Mice

2001; Elsevier BV; Volume: 16; Issue: 2 Linguagem: Inglês

10.1006/jaut.2000.0470

1095-9157

Yasunori Kageyama, Yukio Koide, Toshi Nagata, Masato Uchijima, Atsushi Yoshida, Tomio Arai, Takayuki Miura, Cristina Miyamoto, Akira Nagano,

Cellular Mechanics and Interactions

The aim of this study was to explore the roles of toxic shock syndrome toxin-1 (TSST-1) in collagen-induced arthritis (CIA). DBA/1 mice were immunized with type II collagen (CII) and treated with TSST-1. Intraperitoneal and intravenous injections of TSST-1 aggravated CIA, enhancing its incidence and severity. CIA was accompanied by an increase in anti-CII IgG Ab levels. Intraperitoneal administration with TSST-1 enhanced IFN-gamma, TNF-alpha, and IL-4 production in DBA/1 mice. We discovered the mRNA expressions of IFN-gamma, IL-2, TNF-alpha, IL-1beta, and iNOS in spleen cells stimulated with TSST-1 in vitro. However, IL-12 and IL-4 mRNA expression were seen constitutively without stimulation. Only a little increase of IL-12 and IL-4 mRNA expression was seen at 2-3 h after treatment with TSST-1. Our experiments demonstrated that CIA was aggravated by the treatment with TSST-1, which may have induced various proinflammatory cytokines and the production of both Th1 and Th2 cytokines.