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Neurogenesis in dentate subgranular zone and rostral subventricular zone after focal cerebral ischemia in the rat

2001; National Academy of Sciences; Volume: 98; Issue: 8 Linguagem: Inglês

10.1073/pnas.081011098

1091-6490

Kunlin Jin, Manabu Minami, Jing Lan, Xiao Mao, Sophie Batteur, Roger P. Simon, David A. Greenberg,

Zebrafish Biomedical Research Applications

Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating cells were labeled with 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdUrd) over 2-day periods before sacrificing animals 1, 2 or 3 weeks after ischemia. Ischemia increased the incorporation of BrdUrd into cells in two neuroproliferative regions-the subgranular zone of the dentate gyrus and the rostral subventricular zone. Both effects were bilateral, but that in the subgranular zone was more prominent on the ischemic side. Cells labeled with BrdUrd coexpressed the immature neuronal markers doublecortin and proliferating cell nuclear antigen but did not express the more mature cell markers NeuN and Hu, suggesting that they were nascent neurons. These results support a role for ischemia-induced neurogenesis in what may be adaptive processes that contribute to recovery after stroke.