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Association of Tumor Necrosis Factor-.ALPHA. with Fever and Pulmonary Lesion Score in Pigs Experimentally Infected with Swine Influenza Virus Subtype H1N2

2009; Japanese Society of Veterinary Science; Volume: 71; Issue: 5 Linguagem: Inglês

10.1292/jvms.71.611

1347-7439

Bongtae Kim, Kyoung Kyu Ahn, Yoon‐Cheol Ha, Yong Hoon Lee, Duyeol Kim, Jeong Han Lim, Sung‐Hoon Kim, Miyoung Kim, Kyung-Dong Cho, Bog‐Hieu Lee, Chanhee Chae,

Respiratory viral infections research

The relationship between tumor necrosis factor (TNF)-alpha level, body temperature, and pulmonary lesion score was determined in 3-week-old pigs inoculated intranasally with swine influenza virus (SIV) subtype H1N2. The expression of TNF-alpha was measured in bronchoalveolar lavage (BAL) fluids by enzyme-linked immunosorbent assay and lung tissues by immunohistochemistry. In BAL fluid, TNF-alpha concentration was maximal at 1 days post-inoculation (dpi), declined markedly by 3 dpi (P<0.05) and steady thereafter. Mean rectal temperatures were above 40 degrees C for the infected groups at 1 dpi and declined markedly by 3 dpi. The body temperatures were correlated with the levels of TNF-alpha in BAL fluids from pigs experimentally infected with SIV (r(s)=0.9754, P<0.05). The pulmonary lesion scores were correlated with the means of positive cells by immunohistochemistry for TNF- alpha (r(s)=0.9949, P<0.001). The results suggest that the expression of TNF-alpha protein plays an important role in induction of pulmonary lesion and clinical sign such as fever in SIV infection.