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Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

2011; Research Foundation of Ribeirão Preto; Volume: 10; Issue: 1 Linguagem: Inglês

10.4238/vol10-1gmr1154

1676-5680

Sumon Ghosh, Prasanta Majumder, Swati Sen Mandi,

Ethnobotanical and Medicinal Plants Studies

The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia.Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties.The most important species of this genus, with maximal therapeutic properties, is Zingiber officinale (garden ginger), which is often adulterated with other lesspotent Zingiber sp.There is an existing demand in the herbal drug industry for an authentication system for the Zingiber sp in order to facilitate their commercial use as genuine phytoceuticals.To this end, we used amplified fragment length polymorphism (AFLP) to produce DNA fingerprints for three Zingiber species.Sixteen collections (six of Z. officinale, five of Z. montanum, and five of Z. zerumbet) were used in the study.Seven selective primer pairs were found to be useful for all the accessions.A total of 837 fragments were produced by these primer pairs.Species-specific markers were identified for all three Zingiber species (91 for Z. officinale, 82 for Z. montanum, and 55 for Z. zerumbet).The dendogram analysis generated from AFLP patterns showed that Z. montanum and Z. zerumbet are phylogenetically closer to each other than to Z. officinale.The AFLP fingerprints of the Zingiber species could be ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 10 (1): 218-229 (2011) AFLP markers for identification of three Zingiber species used to authenticate Zingiber sp-derived drugs and to resolve adulterationrelated problems faced by the commercial users of these herbs.