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Sensitive and Visual Detection of Adenosine by a Rationally Designed FokI‐Based Biosensing Strategy

2009; Wiley; Volume: 4; Issue: 9 Linguagem: Inglês

10.1002/asia.200900200

1861-4728

Ming Zhang, Jing Huang, Ming‐Gang Deng, Xiaocheng Weng, Heng Ma, Xiang Zhou,

RNA Interference and Gene Delivery

Eye spy: A FokI based aptamer–DNAzyme biosensor, in which the aptamer is used as the single input device and the DNAzyme as the device for the single output detection, is presented. Furthermore, this assay system is shown to be a sensitive and selective system, suitable for colorimetric and visual operation by the naked eye. The limit of detection (LOD) of 2 μM is obtained for the adenosine assay.