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Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study

2008; Wiley; Volume: 24; Issue: 4 Linguagem: Inglês

10.1111/j.1439-0426.2008.01134.x

1439-0426

Ákos Horváth, William R. Wayman, Jan C. Dean, Béla Urbányi, Terrence R. Tiersch, Steven D. Mims, David Johnson, Jill A. Jenkins,

Reproductive Biology and Fertility

Journal of Applied IchthyologyVolume 24, Issue 4 p. 443-449 Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study Á. Horváth, Á. Horváth Szent István University, Department of Fish Culture, Gödöllő, HungarySearch for more papers by this authorW. R. Wayman, W. R. Wayman Warm Springs Fish Technology Center, U.S. Fish and Wildlife Service, Warm Springs, GA, USASearch for more papers by this authorJ. C. Dean, J. C. Dean Natchitoches National Fish Hatchery, U.S. Fish and Wildlife Service, Natchitoches, LA, USASearch for more papers by this authorB. Urbányi, B. Urbányi Szent István University, Department of Fish Culture, Gödöllő, HungarySearch for more papers by this authorT. R. Tiersch, T. R. Tiersch Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA, USASearch for more papers by this authorS. D. Mims, S. D. Mims Aquaculture Research Center, Kentucky State University, Frankfort, KY, USASearch for more papers by this authorD. Johnson, D. Johnson IAP World Services at National Wetlands Research Center (NWRC), Lafayette, LA, USASearch for more papers by this authorJ. A. Jenkins, J. A. Jenkins U.S. Geological Survey, NWRC, Lafayette, LA, USASearch for more papers by this author Á. Horváth, Á. Horváth Szent István University, Department of Fish Culture, Gödöllő, HungarySearch for more papers by this authorW. R. Wayman, W. R. Wayman Warm Springs Fish Technology Center, U.S. Fish and Wildlife Service, Warm Springs, GA, USASearch for more papers by this authorJ. C. Dean, J. C. Dean Natchitoches National Fish Hatchery, U.S. Fish and Wildlife Service, Natchitoches, LA, USASearch for more papers by this authorB. Urbányi, B. Urbányi Szent István University, Department of Fish Culture, Gödöllő, HungarySearch for more papers by this authorT. R. Tiersch, T. R. Tiersch Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA, USASearch for more papers by this authorS. D. Mims, S. D. Mims Aquaculture Research Center, Kentucky State University, Frankfort, KY, USASearch for more papers by this authorD. Johnson, D. Johnson IAP World Services at National Wetlands Research Center (NWRC), Lafayette, LA, USASearch for more papers by this authorJ. A. Jenkins, J. A. Jenkins U.S. Geological Survey, NWRC, Lafayette, LA, USASearch for more papers by this author First published: 24 July 2008 https://doi.org/10.1111/j.1439-0426.2008.01134.xCitations: 28 Author’s address: Ákos Horváth, Szent István University, Páter K. u. 1., Gödöllő, H-2103, Hungary. E-mail: Horvath.Akos@mkk.szie.hu Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Summary Populations of sturgeon across the globe are threatened due to unregulated harvest and habitat loss, and the status varies among species across North America. Ready access to viable and functional sperm would contribute to recovery programmes for these species. In this study, we examined the motility, viability (cell membrane integrity) of cryopreserved sperm from three North American acipenseriform species and fertilizing capacity. Milt samples were collected from captive shortnose sturgeon (Acipenser brevirostrum), wild paddlefish (Polyodon spathula) and pallid sturgeon (Scaphirhynchus albus) and cryopreserved using combinations of Modified Tsvetkova’s (MT) extender, Original Tsvetkova’s extender, and modified Hanks’ balanced salt solution, along with the cryoprotectants methanol (MeOH) or dimethyl sulfoxide (DMSO). A dual-staining technique using the fluorescent stains SYBR-14 and propidium iodide was employed with flow cytometry to determine the percentages of spermatozoa that were viable by virtue of having intact membranes. The percentage of viable spermatozoa ranged from 5% to 12% in shortnose sturgeon, 30–59% in paddlefish, and 44–58% in pallid sturgeon. In the first experiment with shortnose sturgeon sperm, methanol allowed for higher values for dependent variables than did DMSO, and sperm viability generally correlated with post-thaw motility. However, fertilization rate, neurulation, or hatching rates were independent from these factors. In the second experiment with shortnose sturgeon, 5% MeOH combined with MT yielded higher values for all parameters tested than the other combinations: viability was correlated with motility, fertilization rate, and hatching rate. Overall, viability and post-thaw motility was not affected by the use of hyperosmotic extenders (OT) or cryoprotectants (DMSO), but their use decreased fertilization percentages. For paddlefish sperm (experiment 3), MT combined with 10% MeOH was clearly a good choice for cryopreservation; viability and motility results were correlated, but independent of fertilization. For pallid sturgeon sperm (experiment 4), MT with 5–10% MeOH showed significantly higher sperm quality and fertilization parameters. Membrane integrity can be used as a predictor of fertilization by cryopreserved sperm, however additional sperm quality parameters, supplementary to motility and membrane integrity, would be useful in the refining and optimizing cryopreservation protocols with acipenseriform sperm. Citing Literature Volume24, Issue4August 2008Pages 443-449 RelatedInformation