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BIBTEX RIS

A New Method for Sampling Maternal Blood in the Placental Intervillous Space

1995; Karger Publishers; Volume: 10; Issue: 5 Linguagem: Inglês

10.1159/000264252

1421-9964

José Simon Camelo, Francisco Eulógio Martinez, Salim Moysés Jorge, Maria Matheus de Sala,

Neonatal and fetal brain pathology

The intervillous space (IVS) is part of the histofunctional unit of the human placenta and by being a fetal-maternal exchange interface is an important subject of study for the understanding of fetal physiology, especially in nutritional investigations. A method developed for the collection of IVS blood has permitted to evaluate the fetal-maternal exchanges in an effective manner. Two disadvantages of this method, however, are the mixing of IVS blood and fetal blood and marked hemolysis. In the present study we introduce and describe some modifications of this method using a single stylet for the perforation of the chorionic plate which simplifies collection and reduces the chance of mixing and hemolysis of the samples obtained.