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Paradoxical ATP Elevation in Ischemic Penumbra Revealed by Quantitative Imaging Mass Spectrometry

2010; Mary Ann Liebert, Inc.; Volume: 13; Issue: 8 Linguagem: Inglês

10.1089/ars.2010.3290

1557-7716

Katsuji Hattori, Mayumi Kajimura, Takako Hishiki, Tsuyoshi Nakanishi, Akiko Kubo, Yoshiko Nagahata, Mitsuyo Ohmura, Ayako Yachie‐Kinoshita, Tomomi Matsuura, Takayuki Morikawa, Tomomi Nakamura, Mitsutoshi Setou, Makoto Suematsu,

Electrochemical Analysis and Applications

Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges. Antioxid. Redox Signal. 13, 1157–1167.