The Regulation of Skin Pigmentation
2007; Elsevier BV; Volume: 282; Issue: 38 Linguagem: Inglês
10.1074/jbc.r700026200
ISSN1083-351X
AutoresYuji Yamaguchi, Michaela Brenner, Vincent J. Hearing,
Tópico(s)Olfactory and Sensory Function Studies
ResumoVisible pigmentation of the skin, hair, and eyes depends primarily on the functions of melanocytes, a very minor population of cells that specialize in the synthesis and distribution of the pigmented biopolymer melanin. Melanocytes are derived from precursor cells (called melanoblasts) during embryological development, and melanoblasts destined for the skin originate from the neural crest. The accurate migration, distribution, and functioning of melanoblasts/melanocytes determine the visible phenotype of organisms ranging from simple fungi to the most complex animal species. In human skin, melanocytes are localized at the dermal/epidermal border in a characteristic regularly dispersed pattern. Each melanocyte at the basal layer of the epidermis is functionally connected to underlying fibroblasts in the dermis and to keratinocytes in the overlying epidermis. Those three types of cells are highly interactive and communicate with each other via secreted factors and their receptors and via cell/cell contacts to regulate the function and phenotype of the skin.Overview: Architecture of the SkinEpidermal melanocytes occur at an approximate ratio of 1:10 among basal keratinocytes and distribute the melanin they produce to ∼40 overlying suprabasal keratinocytes via their elongated dendrites and cell/cell contacts (presented schematically in Fig. 1). Although melanocytes and stem cell keratinocytes in the basal layer of the epidermis are very stable populations that proliferate extremely slowly under normal circumstances, keratinocytes in the upper layers of the epidermis proliferate relatively rapidly. That upward pressure carries them toward the surface of the skin along with their ingested melanin to form a critical barrier for the organism against the environment and the many stresses that originate there. Thus it is not the melanin within melanocytes only, but in combination with the pigment in more superficial layers, that gives skin its characteristic color. Although melanocytes in other locations of the body (e.g. hair follicles, eyes, inner ear, etc.) interact with surrounding cells in manners distinct from those in the epidermis, the basic processes involved in producing the melanin and the organelles within which it is synthesized (termed melanosomes) are comparable, as are the factors that regulate melanogenesis. This review will restrict itself to epidermal pigmentation, and readers interested in factors influencing pigmentation at other sites should consult recent reviews (1Bharti K. Nguyen M.T. Skuntz S. Bertuzzi S. Arnheiter H. Pigment Cell Res. 2006; 19: 380-394Crossref PubMed Scopus (164) Google Scholar, 2Lavado A. Montoliu L. Front. Biosci. 2006; 11: 743-752Crossref PubMed Scopus (21) Google Scholar, 3Sturm R.A. Trends Genet. 2006; 22: 464-468Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar, 4Slominski A. Wortsman J. Plonka P.M. Schallreuter K.U. Paus R. Tobin D.J. J. Investig. Dermatol. 2005; 124: 13-21Abstract Full Text Full Text PDF PubMed Scopus (369) Google Scholar, 5Tobin D.J. Hordinsky M. Bernard B.A. J. Investig. Dermatol. Symp. Proc. 2005; 10: 275-279Abstract Full Text Full Text PDF PubMed Scopus (14) Google Scholar, 6Hornyak T.J. Adv. Dermatol. 2006; 22: 201-218Crossref PubMed Scopus (20) Google Scholar) and books (7Hearing V.J. Leong S.P.L. From Melanocyte to Melanoma: The Progression to Malignancy. Humana Press, New York2005Google Scholar, 8Nordlund J.J. Boissy R.E. Hearing V.J. Oetting W.S. King R.A. Ortonne J.P. The Pigmentary System: Physiology and Pathophysiology. 2006; Crossref Scopus (0) Google Scholar) on those topics.Biochemical ConsiderationsAt this time, more than 125 distinct genes are known that regulate pigmentation either directly or indirectly (9Bennett D.C. Lamoreux M.L. Pigment Cell Res. 2003; 16: 333-344Crossref PubMed Scopus (399) Google Scholar). Many of those affect developmental processes critical to melanoblasts, others regulate the differentiation, survival, etc. of melanocytes, and yet others regulate distinct processes that affect pigmentation. Many of those genes (>25 at latest count) affect the biogenesis or function of melanosomes, the discrete membrane-bound organelles within which melanins are synthesized. Melanosomes, which are closely related to lysosomes and are within the family of lysosome-relatedg organelles (LROs), 3The abbreviations used are: LROlysosome-related organelleACTHadrenocorticotropic hormoneASPAgouti signal proteinDCTDOPAchrome tautomeraseDKKDickkopfDHI5,6-dihydroxyindoleDHICADHI-2-carboxylic acidDOPAl-3,4-dihydroxyphenylalanineEMIepithelial-mesenchymal interactionsEMTepithelial-mesenchymal transitionsbFGFbasic fibroblast growth factorHGFhepatocyte growth factorHOXhomeoboxMC1Rmelanocortin 1 receptorMITFmicrophthalmia transcription factorαMSHα-melanocyte-stimulating hormoneNGFnerve growth factorPOMCpro-opiomelanocortinSCFstem cell factorTYRtyrosinaseUV ultraviolet radiation. require a number of specific enzymatic and structural proteins to mature and become competent to produce melanin (10Chi A. Valencia J.C. Hu Z.Z. Watabe H. Yamaguchi H. Mangini N. Huang H. Canfield V.A. Cheng K.C. Yang F. Abe R. Yamagishi S. Shabanowitz J. Hearing V.J. Wu C.H. Appella E. Hunt D.F. J. Proteome Res. 2006; 5: 3135-3144Crossref PubMed Scopus (150) Google Scholar, 11Hu Z. Valencia J.C. Huang H. Chi A. Shabanowitz J. Hearing V.J. Appella E. Wu C. Int. J. Mass Spectrom. 2007; 259: 147-160Crossref PubMed Scopus (39) Google Scholar). As space does not allow a full consideration of melanosome biogenesis and the specific functions of melanosomal proteins, readers are referred to several recent reviews on this topic (12Kushimoto T. Valencia J.C. Costin G.E. Toyofuku K. Watabe H. Yasumoto K. Rouzaud F. Vieira W.D. Hearing V.J. Pigment Cell Res. 2003; 16: 237-244Crossref PubMed Scopus (72) Google Scholar, 13Barral D.C. Seabra M.C. Pigment Cell Res. 2004; 17: 111-118Crossref PubMed Scopus (97) Google Scholar). Suffice it to say that the critical enzymes include tyrosinase (TYR), Tyrp1, and Dct, mutations of which dramatically affect the quantity and quality of melanins synthesized. Critical structural proteins include Pmel17 (also known as gp100) and MART1, both of which are required for the structural maturation of melanosomes. A large number of proteins are involved in the sorting/trafficking of proteins to melanosomes, and mutations in any of those typically lead to inherited hypopigmentary disorders (8Nordlund J.J. Boissy R.E. Hearing V.J. Oetting W.S. King R.A. Ortonne J.P. The Pigmentary System: Physiology and Pathophysiology. 2006; Crossref Scopus (0) Google Scholar). Melanocytes can produce three distinct kinds of melanins: two types of eumelanin, which are the predominant pigments found in dark skin and black hair, and pheomelanin, which is associated with the red hair/freckled skin phenotype. As melanosomes mature and their constituent proteins are delivered, the organelles themselves become cargos carried by various molecular motors from the perinuclear area to the cell periphery (14Coudrier E. Pigment Cell Res. 2007; 20: 153-160Crossref PubMed Scopus (20) Google Scholar, 15Wu X. Hammer J.A. II I Pigment Cell Res. 2000; 13: 241-247Crossref PubMed Scopus (41) Google Scholar), after which they are transferred to neighboring keratinocytes.The type(s) of melanin produced depends on the function of melanogenic enzymes and the availability of substrates. Biosynthesis of melanin depends on TYR, and mutations disrupting TYR function result in an inherited pigmentary disorder known as albinism. TYR performs the critical rate-limiting activity of hydroxylating tyrosine to l-3,4-dihydroxyphenylalanine (DOPA), which is rapidly converted to DOPAquinone. If cysteine is available, it will stoichiometrically react with nascent DOPAquinone to yield 3- or 5-cysteinyl DOPAs, which then oxidize and polymerize, giving rise to yellow-red soluble melanins known collectively as pheomelanins (16Hennessy A. Oh C. Diffey B. Wakamatsu K. Ito S. Rees J.L. Pigment Cell Res. 2005; 18: 220-223Crossref PubMed Scopus (108) Google Scholar, 17Liu Y. Hong L. Wakamatsu K. Ito S. Adhyaru B. Cheng C.Y. Bowers C.R. Simon J.D. Photochem. Photobiol. 2005; 81: 135-144Crossref PubMed Scopus (156) Google Scholar). As intramelanosomal cysteine is depleted, the excess DOPAquinone spontaneously cyclizes to form an orange intermediate known as DOPAchrome. The carboxylic acid of DOPAchrome will be spontaneously lost generating 5,6-dihydroxyindole (DHI), which rapidly oxidizes and polymerizes to form dark brown/black, high molecular weight insoluble polymers, known as DHI-melanin. However, if DOPAchrome tautomerase (DCT) is present, DOPAchrome will tautomerize without losing its carboxylic acid group to form DHI-2-carboxylic acid (DHICA), which can oxidize and polymerize to form yet a third type of melanin, known as DHICA-melanin, which is a lighter brown color, moderately soluble and of intermediate size (18Ito S. Pigment Cell Res. 2003; 16: 230-236Crossref PubMed Scopus (371) Google Scholar). Human skin normally contains mixtures of all three types of melanins, and the ratio of those in part determines visible pigmentation (19Wakamatsu K. Kavanagh R. Kadekaro A.L. Terzieva S. Sturm R.A. Leachman S. Abdel-Malek Z. Ito S. Pigment Cell Res. 2006; 19: 154-162Crossref PubMed Scopus (100) Google Scholar).Developmental ConsiderationsMelanoblasts originating in the neural crest must develop, migrate to appropriate sites, survive, differentiate to melanocytes, and then function to produce normal pigmentation patterns. A large number of genes (>25) are known to be involved in those processes, mutations in which cause developmental pigmentary diseases. In addition to genes expressed by melanocytes, signaling factors originating from adjacent tissues play critical roles in guiding those processes. Epithelial-mesenchymal interactions (EMI) refer to proximate paracrine or juxtacrine cross-talk between stromal fibroblasts and tissue epithelia and differ from epithelial-mesenchymal transitions (EMT), which refer to the transdifferentiation of epithelial cells to a fibroblast-like phenotype. EMI as well as EMT is required for the development of various organs; the key signaling pathways involved in EMI include homeobox (HOX), fibroblast growth factors, sonic hedgehogs, Wnt/β-catenin/Lef1, and bone morphogenesis proteins. EMI also play crucial roles in skin development and in melanocyte development/function as well. HOX genes influence the normal development of skin appendages, the pigmentary system, and stratified epidermis during embryogenesis. Transgenic and knock-out mice studies reveal that not only HOX genes but also the Wnt/β-catenin/Lef1 signaling pathways are involved in melanoblast development. Taken together, EMI are indispensable for site-specific organogenesis including liver, lung, kidney, peripheral nerves, digits, skin, and skin appendages. Readers are referred to recent reviews on this topic for further information (6Hornyak T.J. Adv. Dermatol. 2006; 22: 201-218Crossref PubMed Scopus (20) Google Scholar, 7Hearing V.J. Leong S.P.L. From Melanocyte to Melanoma: The Progression to Malignancy. Humana Press, New York2005Google Scholar, 8Nordlund J.J. Boissy R.E. Hearing V.J. Oetting W.S. King R.A. Ortonne J.P. The Pigmentary System: Physiology and Pathophysiology. 2006; Crossref Scopus (0) Google Scholar, 20Levy C. Khaled M. Fisher D.E. Trends Mol. Med. 2006; 12: 406-414Abstract Full Text Full Text PDF PubMed Scopus (810) Google Scholar).Regulation of Constitutive Skin PigmentationSkin colors in humans range from extremely fair/light to extremely dark depending on racial/ethnic background, but the density of melanocytes in a given area (e.g. the back or arms) is virtually identical in all types of skin (21Tadokoro T. Kobayashi N. Zmudzka B.Z. Ito S. Wakamatsu K. Yamaguchi Y. Korossy K.S. Miller S.A. Beer J.Z. Hearing V.J. FASEB J. 2003; 17: 1177-1179Crossref PubMed Scopus (281) Google Scholar). Keratinocytes in fair skin tend to cluster their poorly pigmented melanosomes above the nuclei, whereas in dark skin the heavily pigmented melanosomes are distributed individually in keratinocytes, thus maximizing their absorption of light (shown schematically in Fig. 1). There is a large intra-individual variation in melanocyte density in different areas of the body, e.g. the difference between skin on the palms/soles compared with other areas of the body. Constitutive melanocyte density in the skin can be affected by the environment, e.g. by chronic ultraviolet radiation (UV) (which can increase melanocyte density by 3- or 4-fold) or by toxic compounds such as hydroquinone (which can selectively and permanently destroy melanocytes in the skin). Inherited pigmentary disorders can also result in increased melanocyte density (e.g. freckles) or in decreased melanocyte density (e.g. vitiligo). (An excellent resource for pigmentary genes, their functions, and their involvement in pigmentary diseases can be found at ifpcs.med.umn.edu/micemut.htm.)Epidermal melanocytes proliferate slowly, if at all, under normal circumstances, and they are quite resistant to apoptosis because of their high expression of Bcl2 (22McGill G.G. Horstmann M.A. Widlund H.R. Du J. Motyckova G. Nishimura E. Lin Y.L. Ramaswamy S. Avery W. Ding H.F. Jordan S.A. Jackson I.J. Korsmeyer S. Golub T.R. Fisher D.E. Cell. 2002; 109: 707-718Abstract Full Text Full Text PDF PubMed Scopus (591) Google Scholar). Melanocyte density and differentiation is influenced by the environment, including UV and factors secreted by neighboring keratinocytes and fibroblasts (shown schematically in Fig. 2). For example, it was recently shown that fibroblasts in the dermis of the palms/soles secrete high levels of DKK1, which suppresses melanocyte growth and function by inhibiting the Wnt/β-catenin signaling pathway (23Yamaguchi Y. Itami S. Watabe H. Yasumoto K. Abdel-Malek Z.A. Kubo T. Rouzaud F. Tanemura A. Yoshikawa K. Hearing V.J. J. Cell Biol. 2004; 165: 275-285Crossref PubMed Scopus (180) Google Scholar, 24Yamaguchi Y. Passeron T. Watabe H. Yasumoto K. Rouzaud F. Hoashi T. Hearing V.J. J. Investig. Dermatol. 2007; 127: 1217-1225Abstract Full Text Full Text PDF PubMed Scopus (80) Google Scholar). DKK1 inhibition of Wnt signaling in melanocytes dramatically inhibits the melanogenic pathway, ranging from effects on transcriptional regulators (such as MITF) to downstream melanogenic proteins. DKK1 also affects keratinocytes in overlying epidermis, reducing their uptake of melanin and inducing a thicker less pigmented skin phenotype. 4Y. Yamaguchi, T. Passeron, H. Watabe, F. Rouzaud, K. Yasumoto, T. Hara, T. Miki, and V. J. Hearing, submitted for publication. The dermis in adult skin retains the expression patterns of HOX genes (25Chang H.Y. Chi J.T. Dudoit S. Bondre C. van de Rijn M. Botstein D. Brown P.O. Proc. Natl. Acad. Sci. U. S. A. 2002; 99: 12877-12882Crossref PubMed Scopus (839) Google Scholar), which regulate patterning in primary and secondary axes of the embryo, suggesting that HOX genes regulate site-specific homeostasis even in adult tissue. This finding implies that an upstream regulator of DKK1 may be a specific HOX gene.FIGURE 2Schematic of receptors, ligands, and other factors that regulate pigmentation of human skin. Abbreviations for most of these factors, receptors, proteins, and genes are given in Footnote 3.View Large Image Figure ViewerDownload Hi-res image Download (PPT)One major determinant of pigment phenotype of the skin is the melanocortin 1 receptor (MC1R), a G protein-coupled receptor that regulates the quantity and quality of melanins produced (26Rees J.L. Pigment Cell Res. 2000; 13: 135-140Crossref PubMed Scopus (156) Google Scholar, 27Lin J.Y. Fisher D.E. Nature. 2007; 445: 843-850Crossref PubMed Scopus (823) Google Scholar). MC1R function is controlled by the agonists α-melanocyte-stimulating hormone (αMSH) and adrenocorticotropic hormone (ACTH) and by an antagonist, Agouti signaling protein (ASP). Activation of the MC1R by an agonist stimulates the expression of the melanogenic cascade and thus the synthesis of eumelanin, whereas ASP can reverse those effects and elicit the production of pheomelanin. αMSH and ACTH can also up-regulate expression of the MC1R gene, thus acting in a positive feedback loop. MC1R function controls the switch to produce eu- versus pheomelanin, but the mechanism(s) underlying that switch remains unknown.In sum, constitutive skin pigmentation is determined by: (a) the migration of melanoblasts to that tissue during development, (b) their survival and differentiation to melanocytes, (c) the density of melanocytes, (d) the expression/function of enzymatic and structural constituents of melanosomes, (e) the synthesis of different types of melanin (eu- and pheomelanin), (f) the transport of melanosomes to dendrites, (g) the transfer of melanosomes to keratinocytes, and finally (h) the distribution of melanin in suprabasal layers of the skin.Regulation of Facultative Skin PigmentationFacultative skin pigmentation is the term coined for increased skin color due to some type of physiological regulation. Many factors regulate constitutive skin color, the most obvious of them being UV in what is commonly termed the tanning reaction (28Eller M.S. Gilchrest B.A. Pigment Cell Res. 2000; 13: 94-97Crossref PubMed Scopus (74) Google Scholar, 29Tadokoro T. Yamaguchi Y. Batzer J. Coelho S.G. Zmudzka B.Z. Miller S.A. Wolber R. Beer J.Z. Hearing V.J. J. Investig. Dermatol. 2005; 124: 1326-1332Abstract Full Text Full Text PDF PubMed Scopus (185) Google Scholar). Recent studies have outlined the complex kinetics of responses of the skin to UV radiation, which result in tanning over the course of several weeks (30Miyamura Y. Coelho S.G. Wolber R. Miller S.A. Wakamatsu K. Zmudzka B.Z. Ito S. Smuda C. Passeron T. Choi W. Batzer J. Yamaguchi Y. Beer J.Z. Hearing V.J. Pigment Cell Res. 2007; 20: 2-13Crossref PubMed Scopus (170) Google Scholar).UV radiation is the most significant factor influencing human skin pigmentation. As a direct effect of UV, especially UVA, immediate pigment darkening occurs within minutes and persists for several hours followed by persistent pigment darkening, which occurs within several hours and lasts for several days (31Young A.R. Prog. Biophys. Mol. Biol. 2006; 92: 80-85Crossref PubMed Scopus (171) Google Scholar). These rapid increases in pigmentation do not result from acute melanin synthesis but rather from the oxidation and polymerization of existing melanin and the redistribution of existing melanosomes. Delayed tanning also occurs several days after UV exposure but takes longer because it involves the activation of melanocyte function. Exposure to UV leads to increased expression of MITF (the master transcriptional regulator of pigmentation) and its downstream melanogenic proteins, including Pmel17, MART-1, TYR, TRP1, and DCT (30Miyamura Y. Coelho S.G. Wolber R. Miller S.A. Wakamatsu K. Zmudzka B.Z. Ito S. Smuda C. Passeron T. Choi W. Batzer J. Yamaguchi Y. Beer J.Z. Hearing V.J. Pigment Cell Res. 2007; 20: 2-13Crossref PubMed Scopus (170) Google Scholar, 32Yamaguchi Y. Hearing V.J. Hearing V.J. Leong S.P.L. From Melanocytes to Malignant Melanoma. 2005: 101-118Google Scholar), leading eventually to increases in melanin content. Increased levels of PAR2 in keratinocytes also result from exposure to UV, which increases uptake and distribution of melanosomes by keratinocytes in the epidermis (33Scott G. Deng A. Rodriguez-Burford C. Seiberg M. Han R. Babiarz L. Grizzle W. Bell W. Pentland A. J. Investig. Dermatol. 2001; 117: 1412-1420Abstract Full Text Full Text PDF PubMed Scopus (93) Google Scholar).Epidermal melanocytes and keratinocytes also respond to UV exposure by increasing their expression of αMSH and ACTH, which up-regulates the expression and function of MC1R and consequently enhances melanocyte responses to those melanocortins. Variants of MC1R that function weakly are found in individuals with red hair and fair skin who contain predominantly pheomelanin and have a relative inability to tan. Endothelin 1 expression by keratinocytes is also increased by UV and enhances the expression of MC1R, although it works through its own receptor (EDNRB) on melanocytes. Interleukin-1 secretion by keratinocytes is also elicited by UV and it stimulates the secretion of ACTH, αMSH, endothelin 1, and bFGF by keratinocytes. Other melanogenic factors produced by keratinocytes in response to UV include SCF and NGF. The tanning response also relies on stimulation of secretion of NGF by keratinocytes, which prevents melanocyte apoptotic cell death following UV exposure (34Kadekaro A.L. Kavanagh R. Wakamatsu K. Ito S. Pipitone M.A. Abdel-Malek Z.A. Pigment Cell Res. 2003; 16: 434-447Crossref PubMed Scopus (144) Google Scholar). Stimulation of p53 in keratinocytes by UV increases expression of the POMC gene, leading to increased secretion of αMSH and stimulation of MC1R function in neighboring melanocytes (35Cui R. Widlund H.R. Feige E. Lin J.Y. Wilensky D.L. Igras V.E. D'Orazio J.A. Fung C.Y. Schanbacher C.F. Granter S.R. Fisher D.E. Cell. 2007; 128: 853-864Abstract Full Text Full Text PDF PubMed Scopus (471) Google Scholar). UV can also affect fibroblasts in the dermis; growth factors secreted from those cells in response to UV include HGF, bFGF, and SCF, all factors that stimulate pigmentation via their receptors on melanocytes (36Imokawa G. Pigment Cell Res. 2004; 17: 96-110Crossref PubMed Scopus (301) Google Scholar). Retinoic acid up-regulates the differentiation (i.e. melanogenesis) and proliferation of mammalian melanocytes (37Huang Y. Boskovic G. Niles R.M. J. Cell. Physiol. 2003; 194: 162-170Crossref PubMed Scopus (20) Google Scholar), an effect that seems to be mediated through increased expression of melanocortin receptors.Role of Melanin in Photoprotection of the SkinLightly pigmented skin has a dramatically increased risk of skin cancers, including melanomas, much higher (15–70-fold) than in darker skin (38Gilchrest B.A. Eller M.S. Geller A.C. Yaar M. N. Engl. J. Med. 1999; 340: 1341-1348Crossref PubMed Scopus (663) Google Scholar, 39Landi M.T. Baccarelli A. Tarone R.E. Pesatori A. Tucker M.A. Hedayati M. Grossman L. J. Natl. Cancer Inst. 2003; 94: 94-101Crossref Scopus (84) Google Scholar). Because skin pigmentation is primarily regulated by the MC1R, its gene is considered a susceptibility gene for melanoma (40Robinson S.J. Healy E. Oncogene. 2002; 21: 8037-8046Crossref PubMed Scopus (51) Google Scholar).UV is harmful to human skin because of its production of various types of cellular damage, most notably oxidative damage and two major types of DNA damage: cyclobutane pyrimidine dimers and 6,4-photoproducts (41Agar N. Young A.R. Mutat. Res. 2005; 571: 121-132Crossref PubMed Scopus (196) Google Scholar). Such molecular lesions have significant long-term effects on tissue if not repaired quickly and correctly. There is increasing evidence that DNA damage/repair itself can induce skin pigmentation. Small DNA fragments, such as thymine dinucleotides, enhance pigmentation of melanocytic cells and can stimulate TYR mRNA levels and responses to MSH (42Eller M.S. Ostrom K. Gilchrest B.A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 1087-1092Crossref PubMed Scopus (240) Google Scholar). p53, which regulates the cell cycle and the repair of DNA damage, as well as the induction of apoptosis (32Yamaguchi Y. Hearing V.J. Hearing V.J. Leong S.P.L. From Melanocytes to Malignant Melanoma. 2005: 101-118Google Scholar), can also up-regulate POMC/MSH expression by keratinocytes in response to UV, thereby inducing pigmentation (35Cui R. Widlund H.R. Feige E. Lin J.Y. Wilensky D.L. Igras V.E. D'Orazio J.A. Fung C.Y. Schanbacher C.F. Granter S.R. Fisher D.E. Cell. 2007; 128: 853-864Abstract Full Text Full Text PDF PubMed Scopus (471) Google Scholar).The involvement of MC1R with UV induction of skin pigmentation is complex and is regulated at many levels (43Rouzaud F. Costin G.E. Yamaguchi Y. Valencia J.C. Berens W. Chen K. Hoashi T. Bohm M. Abdel-Malek Z.A. Hearing V.J. FASEB J. 2006; 20: 1927-1929Crossref PubMed Scopus (39) Google Scholar). MC1R regulates melanocyte function primarily via MITF, which in turn regulates melanogenesis and dendricity. MITF expression is stimulated relatively quickly, and significant increases are seen within 1 day of UV exposure. The downstream targets of MITF, e.g. TYR, Pmel17, and DCT, respond more slowly and reach maxima from 1–3 weeks after UV exposure. It takes several weeks after UV exposure before significant increases in melanin synthesis or melanocyte density occur. In addition to its role in pigmentation, MC1R regulates many other properties of melanocytes, such as the activation of DNA repair and other anti-photocarcinogenic activities that are important for protection against the deleterious effects of UV (44Kadekaro A.L. Wakamatsu K. Ito S. Abdel-Malek Z.A. Front. Biosci. 2006; 11: 2157-2173Crossref PubMed Scopus (38) Google Scholar). Although UV increases expression of melanogenic genes similarly in skin of different racial/ethnic groups (29Tadokoro T. Yamaguchi Y. Batzer J. Coelho S.G. Zmudzka B.Z. Miller S.A. Wolber R. Beer J.Z. Hearing V.J. J. Investig. Dermatol. 2005; 124: 1326-1332Abstract Full Text Full Text PDF PubMed Scopus (185) Google Scholar), there are some significant differences including melanin redistribution, protection against DNA damage, and induction of apoptosis in melanin-containing keratinocytes (21Tadokoro T. Kobayashi N. Zmudzka B.Z. Ito S. Wakamatsu K. Yamaguchi Y. Korossy K.S. Miller S.A. Beer J.Z. Hearing V.J. FASEB J. 2003; 17: 1177-1179Crossref PubMed Scopus (281) Google Scholar, 45Yamaguchi Y. Takahashi K. Zmudzka B.Z. Kornhauser A. Miller S.A. Tadokoro T. Berens W. Beer J.Z. Hearing V.J. FASEB J. 2006; 20: 1486-1488Crossref PubMed Scopus (158) Google Scholar). UV stimulates the transfer of melanin from the lower epidermis upward and prevents DNA damage in the lower epidermis more significantly in dark skin than in fair skin (29Tadokoro T. Yamaguchi Y. Batzer J. Coelho S.G. Zmudzka B.Z. Miller S.A. Wolber R. Beer J.Z. Hearing V.J. J. Investig. Dermatol. 2005; 124: 1326-1332Abstract Full Text Full Text PDF PubMed Scopus (185) Google Scholar, 45Yamaguchi Y. Takahashi K. Zmudzka B.Z. Kornhauser A. Miller S.A. Tadokoro T. Berens W. Beer J.Z. Hearing V.J. FASEB J. 2006; 20: 1486-1488Crossref PubMed Scopus (158) Google Scholar). UV induces significantly more apoptosis in dark skin than in fair skin, which suggests a more efficient removal of UV-damaged cells; this may play a role in the decreased photocarcinogenesis of darker skin.Disrupted Regulation of Skin PigmentationThe regulation of skin pigmentation sometimes goes awry, leading to pigmentary disorders of many types. Intracellular pH is an important consideration to the regulation of TYR function (46Watabe H. Valencia J.C. Yasumoto K. Kushimoto T. Ando H. Muller J. Vieira W.D. Mizoguchi M. Appella E. Hearing V.J. J. Biol. Chem. 2004; 279: 7971-7981Abstract Full Text Full Text PDF PubMed Scopus (104) Google Scholar), not only because intramelanosomal pH dramatically affects catalytic functions but also because a correct pH gradient is critical to the sorting pathway responsible for delivery of melanosomal proteins (47Watabe H. Valencia J.C. Le Pape E. Yamaguchi Y. Nakamura M. Rouzaud F. Hoashi T. Kawa Y. Mizoguchi M. Hearing V.J. J. Investig. Dermatol. 2007; Google Scholar). There are many instances where normal levels of functional TYR are produced by melanocytic cells and yet little or no melanin is formed; it is even thought that intracellular pH plays an important role in regulating pigment production in various types of skin according to racial/ethnic origin (48Smith D.R. Spaulding D.T. Glenn H.M. Fuller B.B. Exp. Cell Res. 2004; 298: 521-534Crossref PubMed Scopus (81) Google Scholar).TYR function is also regulated by proteasome activity. This occurs during normal pigmentation and is possibly regulated by intracellular levels of fatty acids, but it becomes especially important in the degradation of mutant TYR that occurs in some forms of albinism. The endoplasmic reticulum-associated degradation system is exquisitely sensitive to almost any small perturbation in TYR structure or its chaperone-like protein Tyrp1, leading to extensive hypopigmentation of tissues. Those interested in the mechanisms of hypopigmentation are referred to a recent review of the topic (49Ando H. Kondoh H. Ichihashi M. Hearing V.J. J. Investig. Dermatol. 2007; 127: 751-761Abstract Full Text Full Text PDF PubMed Scopus (262) Google Scholar).All forms of albinism result from the dysfunction of TYR and/or other melanogenic proteins, which leads to impaired pigmentation of the skin, hair and eyes (50King R.A. Hearing V.J. Creel D.J. Oetting W.S. Scriver C.R. Beaudet A.L. Sly W.S. Valle D. The Metabolic and Molecular Bases of Inherited Disease. 2001: 5587-5627Google Scholar). By its nature, only pigmented tissues are affected; to date, five types of albinism have been defined that map to five distinct pigment-related loci. Mutations in any of those genes impact TYR activity either directly or indirectly: oculocutaneous albinism (OCA) type 1 (TYR) and OCA3 (TYRP1) by leading to proteasomal degradation of TYR, OCA2 (P) and OCA4 (MATP) by disrupting the sorting of functional TYR to melanosomes. OA1 (OA1) impairs melanosome biogenesis and pigmentation by an as yet unknown mechanism and thereby disrupts the production of melanin (51Incerti B. Cortese K. Pizzigoni A. Surace E.M. Varani S. Coppola M. Jeffery G. Seeliger M. Jaissle G. Bennett D.C. M
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