Mapping of Nuclear Localization Signals by Simultaneous Fusion to Green Fluorescent Protein and to β-Galactosidase
1999; Future Science Ltd; Volume: 26; Issue: 5 Linguagem: Inglês
10.2144/99265bm12
ISSN1940-9818
AutoresGabriele Sorg, Thomas Stamminger,
Tópico(s)CRISPR and Genetic Engineering
ResumoBioTechniquesVol. 26, No. 5 BenchmarksOpen AccessMapping of Nuclear Localization Signals by Simultaneous Fusion to Green Fluorescent Protein and to β-GalactosidaseGabriele Sorg & Thomas StammingerGabriele SorgUniversität Erlangen-Nürnberg, Erlangen, Germany & Thomas Stamminger*Address correspondence to Dr. Thomas Stamminger, Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Schloßgarten 4, 91054 Erlangen, Germany. Internet: E-mail Address: tsstammi@viro.med.uni-erlangen.deUniversität Erlangen-Nürnberg, Erlangen, GermanyPublished Online:22 Aug 2018https://doi.org/10.2144/99265bm12AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByThe ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways3 July 2020 | Nature Communications, Vol. 11, No. 1Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis24 September 2020 | Nature Communications, Vol. 11, No. 1A novel overlapping NLS/NES region within the PH domain of Rho Guanine Nucleotide Exchange Factor (RGNEF) regulates its nuclear-cytoplasmic localizationEuropean Journal of Cell Biology, Vol. 98, No. 1p120 Catenin-Mediated Stabilization of E-Cadherin Is Essential for Primitive Endoderm Specification24 August 2016 | PLOS Genetics, Vol. 12, No. 8Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a modelAnalytical Biochemistry, Vol. 494The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation17 October 2015 | Nucleic Acids Research, Vol. 110ESCRT-III controls nuclear envelope reformation3 June 2015 | Nature, Vol. 522, No. 7555Characterization of RanBPM Molecular Determinants that Control Its Subcellular Localization6 February 2015 | PLOS ONE, Vol. 10, No. 2Dynamics of re-constitution of the human nuclear proteome after cell division is regulated by NLS-adjacent phosphorylation29 October 2014 | Cell Cycle, Vol. 13, No. 22Using multi-channel level sets to measure the cytoplasmic localization of HCMV pUL97 in GFP-B-gal fusion constructsJournal of Virological Methods, Vol. 199The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteinsBiochimica et Biophysica Acta (BBA) - 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