Gas—liquid chromatographic analysis of hydroxyethyl-starch
1983; Elsevier BV; Volume: 254; Linguagem: Inglês
10.1016/s0021-9673(01)88332-1
ISSN1873-3778
AutoresSum Y. Check, Mai Khuong, Kam Sheung-Tsam, Lai Chii-Ming, Avraham Yacobi, Thomas F. Kalhorn,
Tópico(s)Alcohol Consumption and Health Effects
ResumoA gas—liquid chromatographic (GLC) method for the analysis of hydroxyethyl-starch (HES) is described. This method introduces a Schiff-base formation step to eliminate different anomeric forms of reducing sugars in HES, resulting in only one GLC peak for each monosaccharide constituent. Using this GLC method, the major constituents in HES can be separated and reproducibly quantitated using a conventional column under isothermal conditions in less than 20 min. The distribution of sugar constituents in HES at three different molar substitutions was determined and the major monosubstituted glucose was 2-O-hydroxyethylglucose, followed by 6-O-hydroxyethylglucose and 3-O-hydroxyethylglucose. The synthesis of 2-O-(2′-hydroxyethoxy)ethylglucose is described.
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