Produção Nacional
Revisado por pares
Differentiation of Candida species obtained from nosocomial candidemia using RAPD-PCR technique
2006; Brazilian Society of Tropical Medicine; Volume: 39; Issue: 2 Linguagem: Inglês
10.1590/s0037-86822006000200008
ISSN1678-9849
AutoresHenrique Maia Valério, Rita de Cássia Botelho Weikert-Oliveira, Maria Aparecida de Resende,
Tópico(s)Antimicrobial Resistance in Staphylococcus
ResumoThirteen strains of the genus Candida were isolated from catheter, urine and surgical wounds from individual patients of the Santa Casa de Misericórdia, Belo Horizonte, MG, Brazil. Ten strains were characterized as Candida albicans, two as Candida glabrata, and one as Candida parapsilosis. Isolates were evaluated for molecular relatedness by random amplified polymorphic DNA technique using 15 primers. The analysis of the genomic DNA obtained revealed a low intraspecific polymorphism and did not allow for the differentiation between strains of the same species obtained from distinct clinical sources (catheter, urine and surgical wounds). The RAPD profiles generated were able to differentiate among the species of Candida albicans, Candida parapsilosis and Candida glabrata strains isolated in this study.
Referência(s)