A Golden Gate Modular Cloning Toolbox for Plants
2014; American Chemical Society; Volume: 3; Issue: 11 Linguagem: Inglês
10.1021/sb4001504
ISSN2161-5063
AutoresCarola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones, Nicola J. Patron, Sylvestre Marillonnet,
Tópico(s)CRISPR and Genetic Engineering
ResumoPlant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.
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