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Centrifugal partition chromatography(CPC) as a useful method for determination of partition coefficients between octanol and water.

1987; Pharmaceutical Society of Japan; Volume: 35; Issue: 12 Linguagem: Inglês

10.1248/cpb.35.5010

1347-5223

Hiroshi Terada, Yasutaka Kosuge, Nobuyuki Nakaya, Wataru Murayama, Yoshiaki Nunogaki, Kanichi. Nunogaki,

Analytical Chemistry and Chromatography

Centrifugal partition chromatographies (CPC) of three series of compounds, phenols, benzoic acids and N-phenylanthranilic acids, were performed with acetonitrile as a mobile phase and n-hexane as a stationary phase. Values for the partition coefficient (Pcpc) of these compounds were determined from the ratio of their concentration in the n-hexane phase to that in the acetonitrile phase, and they were compared with the partition coefficient between octanol and water determined by the shaking-flask method (Poct). A significant linear relation was obtained between log Pcpc and log Poct for each series of compounds up to log Poct values of about 6, indicating that CPC is a very useful method for simple and accurate determination of Poct.