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BIBTEX RIS

Comparação do desempenho da lipase de candida rugosa imobilizada em suporte híbrido de polissiloxano-polivinilálcool empregando diferentes metodologias

2008; Brazilian Chemical Society; Volume: 31; Issue: 1 Linguagem: Inglês

10.1590/s0100-40422008000100007

ISSN

1678-7064

Autores

Ariela Veloso de Paula, Ana B. R. Moreira, Luciana Palmeira Braga, Heizir F. de Castro, Laura Maria Bruno,

Tópico(s)

Enzyme Catalysis and Immobilization

Resumo

POLYSILOXANE POLYVINYL ALCOHOL HYBRID SUPPORT USING DIFFERENT METHODOLOGIES.The efficiency for immobilizing microbial Candida rugosa lipase on a hybrid matrix of polysiloxane polyvinyl alcohol, by adsorption, covalent coupling and encapsulation was compared.The activities of immobilized derivatives were evaluated using p-nitrophenylpalmitate (hydrolysis) and butyric acid and butanol (esterification) as substrates.Operational stability and storage tests were also performed.Among the procedures tested, the proposed matrix was efficient for immobilizing C. rugosa lipase by adsorption and covalent coupling techniques and unsuitable for encapsulation purposes.The results reveal that better catalytic properties in both aqueous and organic media were demonstrated by the covalent coupling POS-PVA immobilized lipase, including also satisfactory half-life and good storage stability.

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