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Control of oxidative phosphorylation in AS-30D hepatoma mitochondria

1993; Elsevier BV; Volume: 25; Issue: 3 Linguagem: Inglês

10.1016/0020-711x(93)90627-q

1878-6014

Francisco J. López-Gómez, M.Eugenia Torres-Márquez, Rafael Moreno‐Sánchez,

Adipose Tissue and Metabolism

1. The distribution of control of the rate of state 3 respiration of AS-30D hepatoma mitochondria was determined. 2. The ATP/ADP carrier (flux control coefficient, Ci = 0.70) and the ATP synthase (Ci = 0.19–0.32) were the only steps that exerted significant control on the phosphorylating flux supported by either glutamate + malate, pyruvate + malate, or succinate + rotenone. This is in contrast to liver mitochondria where the control is distributed between several steps. 3. It is suggested that this pattern of control of phosphorylation in hepatoma mitochondria is a consequence of a lower content of adenine nucleotides or a higher content of Mg2+.