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Production and characterization of a monoclonal antibody against bovine haptoglobin and its use in an ELISA

1994; Elsevier BV; Volume: 42; Issue: 2 Linguagem: Inglês

10.1016/0165-2427(94)90006-x

1873-2534

Cynthia L. Sheffield, Carol Kamps‐Holtzapple, John R. DeLoach, L.H. Stanker,

Microbial infections and disease research

Haptoglobin (Hp) is the major acute phase reactant found in cattle. As such, it is an excellent indicator of early disease processes and could be used as a marker for pre-clinical illness in cattle. The production of monoclonal antibodies (mAbs) directed against bovine Hp and bovine hemoglobin is described. The anti-haptoglobin mAbs (Hap1, Hap2, Hap3) and the anti-bovine hemoglobin (Hb) mAb (BoHem1) were characterized and tested for cross-reactivity by means of enzyme-linked immunosorbent assay (ELISA) and immunoblotting analyses. Additionally, the development of an ELISA based on an anti-haptoglobin mAb is discussed.