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Use of biochemical lesions for selection of human cells with hybrid cytoplasms.

1975; National Academy of Sciences; Volume: 72; Issue: 5 Linguagem: Inglês

10.1073/pnas.72.5.1812

1091-6490

Woodring E. Wright, Leonard Hayflick,

Cell death mechanisms and regulation

Techniques for preparing large populations of anucleate cytoplasms from cultured eukaryotic cells have only recently been described. The principal value of anucleate cytoplasms derives from studies that can be done after they are fused to whole cells. Since present methods for the isolation of heterokaryons are unsuitable for the selection of hybrids between whole cells and anucleate cytoplasms (heteroplasmons), a selective system has been developed which is based on the capacity of anucleate cytoplasms containing active enzymes to rescue whole cells poisoned with iodoacetate. Ethidium bromide, a partially effective agent, was used in conjunction with iodoacetate to demonstrate the feasibility of selecting heterokaryons by producing complementary biochemical lesions in the parental cell strains. The potential for artifact in these systems is not, however, entirely precluded.