Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Dynamics of Recombinant hG-CSF in Transgenic Goat: Preliminary Study in the Founder during Hormonally Induced Lactation

2013; Taylor & Francis; Volume: 24; Issue: 1 Linguagem: Inglês

10.1080/10495398.2012.729000

1532-2378

R.R. Moura, Érica S. Albuquerque, Carlos Henrique Sousa de Melo, Agostinho S. Alcântara-Neto, Ríbrio Ivan Tavares Pereira Batista, Diana Célia Sousa Nunes‐Pinheiro, Alexsandra Fernandes Pereira, Dárcio Ítalo Alves Teixeira, Luciana Magalhães Melo, Irina Serova, Lyudmila E. Andreeva, O. L. Serov, Vicente José de Figueirêdo Freitas,

Xenotransplantation and immune response

Abstract This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 µg hG-CSF per mL of milk. Two peaks of serum hG-CSF (3,470 and 7,390 pg/mL) were detected in the first half of the lactation. Outside of the lactation, hG-CSF was absent from serum, indicating no ectopic expression. During the treatment to induce lactation, transgenic female presented increased neutrophil and lymphocyte blood counts when compared to nontransgenic female. Despite transient neutrophilia, serum biochemistry profiles indicated normal liver and renal functions. Thus, transgenic goat expressed hG-CSF in quantities sufficient for a commercial bioreactor and remained clinically healthy. Keywords: GoathG-CSFInduced lactationTransgenesis