Portal de Periódicos da CAPES

Dynamic transcriptome of Schizosaccharomyces pombe shown by RNA-DNA hybrid mapping

2008; Nature Portfolio; Volume: 40; Issue: 8 Linguagem: Inglês

10.1038/ng.196

1546-1718

Natalie Dutrow, David A. Nix, Derick G. Holt, Brett Milash, Brian K. Dalley, Erick M. Westbroek, Timothy J. Parnell, Bradley R. Cairns,

Fungal and yeast genetics research

Bradley Cairns and colleagues report a high-resolution strand-specific transcriptome of the fission yeast Schizosaccharomyces pombe. They survey the transcriptome under multiple growth conditions using an RNA-DNA hybridization mapping (HybMap) technique, and find that most of the euchromatic genome is transcribed. We have determined the high-resolution strand-specific transcriptome of the fission yeast S. pombe under multiple growth conditions using a novel RNA-DNA hybridization mapping (HybMap) technique. HybMap uses an antibody against an RNA-DNA hybrid to detect RNA molecules hybridized to a high-density DNA oligonucleotide tiling microarray. HybMap showed exceptional dynamic range and reproducibility, and allowed us to identify strand-specific coding, noncoding and structural RNAs, as well as previously unknown RNAs conserved in distant yeast species. Notably, we found that virtually the entire euchromatic genome (including intergenics) is transcribed, with heterochromatin dampening intergenic transcription. We identified features including large numbers of condition-specific noncoding RNAs, extensive antisense transcription, new properties of antisense transcripts and induced divergent transcription. Furthermore, our HybMap data informed the efficiency and locations of RNA splicing genome-wide. Finally, we observed strand-specific transcription islands around tRNAs at heterochromatin boundaries inside centromeres. Here, we discuss these new features in terms of organism fitness and transcriptome evolution.