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Metabolic activity of fresh and cryopreserved cynomolgus monkey ( Macaca fascicularis ) hepatocytes

2000; Taylor & Francis; Volume: 30; Issue: 7 Linguagem: Inglês

10.1080/00498250050077986

1366-5928

Nicola J. Hewitt, Thomas M. Fischer, U. Zuehlke, Franz Oesch, Dietmar Utesch,

Hormonal and reproductive studies

1. The effect of cryopreservation on the metabolic capacity of monkey hepatocytes over 4 h in suspension and 24 h in culture was determined. Hepatocytes were diluted in a buffer containing 10% DMSO and frozen in a computer-controlled chamber. 2. Initial ethoxyresorufin and ethoxycoumarin O