A Brg1 Null Mutation in the Mouse Reveals Functional Differences among Mammalian SWI/SNF Complexes
2000; Elsevier BV; Volume: 6; Issue: 6 Linguagem: Inglês
10.1016/s1097-2765(00)00127-1
ISSN1097-4164
AutoresScott J. Bultman, Tom Gebuhr, Della Yee, Christian La Mantia, Jackie Nicholson, Anita C. Gilliam, Filippo Randazzo, Daniel Metzger, Pierre Chambon, Gerald R. Crabtree, Terry Magnuson,
Tópico(s)interferon and immune responses
ResumoAbstract Mammalian SWI/SNF complexes utilize either brahma (Brm) or brahma-related gene 1 (Brg1) catalytic subunits to remodel nucleosomes in an ATP-dependent manner. Brm was previously shown to be dispensable, suggesting that Brm and Brg1 are functionally redundant. To test this hypothesis, we have generated a Brg1 null mutation by gene targeting, and, surprisingly, homozygotes die during the periimplantation stage. Furthermore, blastocyst outgrowth studies indicate that neither the inner cell mass nor trophectoderm survives. However, experiments with other cell types demonstrate that Brg1 is not a general cell survival factor. In addition, Brg1 heterozygotes are predisposed to exencephaly and tumors. These results provide evidence that biochemically similar chromatin-remodeling complexes have dramatically different functions during mammalian development.
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