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Gas chromatographic—mass spectrometric method for the determination of flurazepam and its major metabolites in mouse and rat plasma

1994; Elsevier BV; Volume: 658; Issue: 1 Linguagem: Inglês

10.1016/0378-4347(94)00210-x

1872-812X

DeMing Song, Shide Zhang, K. Kohlhof,

Neurotransmitter Receptor Influence on Behavior

A capillary gas chromatographic—negative chemical ionization (NCI) mass spectrometric method for the determination of flurazepam and its metabolites N-1-hydroxyethyl-flurazepam and N-1-desalkyl-flurazepam in mouse and rat plasma was described. Derivatization of the metabolites of flurazepam with BSTFA allowed a highly stable, accurate, and sensitive GC—MS analysis. The use of a single internal standard (halazepam) for the quantification of all compounds saved cost and time. The detection limits were 0.1 ng/ml for N-hydroxyethyl-flurazepam-TMS (Mr = 404), 0.5 ng/ml for desalkyl-flurazepam-TMS (Mr = 360), and 0.5 ng/ml for flurazepam (Mr = 387) with an injection volume of 1 μl at a signal-to-noise ratio greater than 5. The quantitation limit was set to 10 ng/ml for all compounds.