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STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY*

1964; Wiley; Volume: 113; Issue: 2 Linguagem: Inglês

10.1111/j.1749-6632.1964.tb40705.x

1749-6632

Demetrios A. Rigas, Edward Johnson,

Plant pathogens and resistance mechanisms

Annals of the New York Academy of SciencesVolume 113, Issue 2 p. 800-818 STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY* Demetrios A. Rigas, Demetrios A. Rigas Division of Experimental Medicine, University of Oregon Medical School, Portland, Ore.Search for more papers by this authorEdward A. Johnson, Edward A. Johnson Division of Experimental Medicine, University of Oregon Medical School, Portland, Ore.Search for more papers by this author Demetrios A. Rigas, Demetrios A. Rigas Division of Experimental Medicine, University of Oregon Medical School, Portland, Ore.Search for more papers by this authorEdward A. Johnson, Edward A. Johnson Division of Experimental Medicine, University of Oregon Medical School, Portland, Ore.Search for more papers by this author First published: February 1964 https://doi.org/10.1111/j.1749-6632.1964.tb40705.xCitations: 50 * Supported in part by grants from the U. S. Atomic Energy Commission, Contract AT(45–1)-58; the U. S. P. H. S., National Cancer Institute Grant CY-6109; and the Medical Research Foundation of Oregon. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL References 1 Osborne, T. B., L. B. Mendel & I. F. Harris. 1905. The study of the proteins of the castor bean with special reference to the isolation of ricin. Am. J. Physiol. 14: 259– 286. 2 Li, J. G. & E. E. Osgood. 1949. A method for the rapid separation of leukocytes and nucleated erythrocytes from blood or marrow with a phytohemagglutinin from red beans (Phaseolus vulgaris). Blood. 4: 670– 675. 3 Rigas, D. A. & E. E. Osgood. 1955. Purification and properties of the phytohemagglutinin of Phaseolus vulgaris. J. Biol. Chem. 212: 607– 615. 4 Skoog, W. A. & W. S. Beck. 1956. Studies on the fibrinogen, dextran and phytohemagglutinin methods of isolating leukocytes. Blood. 11: 436– 454. 5 Prager, M. D. & R. J. Spear. 1959. Chromatography of McCaslan pole bean hemagglutinin on DEAE-cellulose. Proc. Soc. Exptl. Biol. Med. 100: 68– 70. 6 Nowell, P. C. 1960. Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes. Cancer Research. 20: 462– 466. 7 Chrustschoff, G. K. & E. A. Berlin. 1935. Cytological investigation on cultures of human blood. J. Genet. 31: 243– 251. 8 Bloom, W. 1938. Tissue culture of blood and blood-forming organs. In Handbook of Hematology. H. Downey, Ed. Hoeber. New York . Pp. 1471– 1585. 9 Allgower, M. 1956. The cellular basis of wound repair. Charles C Thomas. Springfield , Ill . 10 Osgood, E. E. 1957. Control of peripheral concentration of leukocytes. In Homeostatic Mechanisms. H. Quastler, Ed. Brookhaven Symposia in Biology. 10: 31– 51. 11 Paul, J. 1958. Establishment of permanent cell strains from human adult peripheral blood. Nature (London). 182: 808. 12 Berman, L. & C. S. Stulberg. 1958. The epithelial like cells from nonleukemic peripheral blood. Blood. 13: 1149– 1167. 13 Bond, V. P., T. M. Fliedner, E. P. Cronkite, J. R. Rubini, G. Brecher & P. K. Schork. 1959. Proliferative potentials of bone marrow and blood cells studied by in vitro uptake of H3-thymidine. Acta Haematol. 21: 1– 15. 14 Petrakis, N. L. & G. Politis. 1962. Prolonged survival of viable mitotically competent mononuclear leukocytes in stored whole blood. New Engl. J. Med. 267: 286– 289. 15 Carstairs, K. 1962. The human small lymphocyte: its possible pluripotential quality. Lancet. 1: 829– 832. 16 Pearmain, G., R. R. Lycette & P. H. Fitzgerald. 1963. Tuberculin-induced mitosis in peripheral blood leukocytes. Lancet. 1: 637– 638. 17 Shrek, R. & S. S. Stefani. 1963. Lymphocytic and intradermal reactions to phytohemagglutinin. Fed. Proc. 22: 428. 18 Bain, B., M. Vas & L. Lowenstein. 1963. A reaction between leukocytes in mixed peripheral blood cultures. Fed. Proc. 22: 428. 19 Moorhead, P. S., P. C. Nowell, W. J. Mellman, D. M. Battips & D. A. Hungerford. 1960. Chromosome preparations of leukocytes cultured from human peripheral blood. Exptl. Cell Research. 20: 613– 616. 20 Nowell, P. C. 1961. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Research. 21: 1518– 1521. 21 Kosenow, W. & R. A. Pfeiffer. 1961. Chromosomenstudien an kurzzeitigen leukocytenkulturen aus peripherem blut. Klin. Wochschr. 39: 151– 152. 22 Becak, W. 1961. Human chromosomes in short-term cultures from peripheric blood leukocytes. Rev. brasil. biol. 21: 281– 286. 23 De grouchy, J. & M. Lamy. 1961. Study of human chromosomes from blood leukocytes. Rev. franç. étude clin. biol. 6: 825– 827. 24 Favier, Y., M. Viette, M. Saint-paul & J. F. Duplan. 1961. Méthode simplifiée de culture de leucocytes a partir de sang humain. Influence de la phaséoline. Nouv. rev. franç. hématol. 1: 872– 879. 25 Saint-paul, M. & Y. Favier. 1961. Contribution à l'étude de l'action des extraits hémagglutinants de Phaseolus vulgaris sur les tissus animaux et vegetaux. Proc. Congr. European Soc. Haematol. 8(II): (No. 495) 2. 26 Hastings, J., S. Freedman, O. Rendon, H. L. Cooper & K. Hirschhorn. 1961. Culture of human white cells using differential leukocyte separation. Nature. 192: 1214– 1215. 27 Jackson, J. F. 1962. Leukocyte cultures in cytogenetics. Bull. Tulane Univ. Med. Fac. 21: 103– 105. 28 Nowell, P. C. 1962. Mitotic inhibition by epinephrine in leukocyte cultures. Fed. Proc. 21: 73. 29 Bender, M. A. & D. M. Prescott. 1962. DNA synthesis and mitosis in cultures of human peripheral leukocytes. Exptl. Cell Research. 27: 221– 229. 30 Mackinney, A. A., JR., F. Stohlman, JR. & G. Brecher. 1962. The kinetics of cell proliferation in cultures of human peripheral blood. Blood. 19: 349– 358. 31 Mcintyre, O. R. & F. G. Ebaugh, JR. 1962. The effect of phytohemagglutinin on leukocyte cultures as measured by P32 incorporation in the DNA, RNA, and acid soluble fractions. Blood. 19: 443– 453. 32 Turner, B., G. M. DEN Dulk & W. C. Thompson. 1962. A technique of chromosome analysis on cultured peripheral blood leukocytes. Med. J. Australia. 49: 893– 894. 33 De la chappelle, A. 1962. Chromosome studies with leukocytes as the initial material. Nord. Med. 68: 1247– 1250. 34 Rubini, J. R. 1963. A proposed mechanism of action of phytohemagglutinin (PHG). Clin. Research. 11: 199. 35 Smithies, O. 1955. Zone electrophoresis in starch gels: Group variations in the serum proteins of normal human adults. Biochem. J. 61: 629– 641. 36 Huisman, T. H. J. 1962. Personal communication. 37 Hennessey, M. A., A. M. Waltersdorph, F. M. Huennekens & B. W. Gabrio. 1962. Erythrocyte metabolism. VI. Separation of erythrocyte enzymes from hemoglobin. J. Clin. Invest. 41: 1257– 1262. 38 Rigas, D. A. An automatic pressure-vacuum ultrafiltration apparatus. In preparation. 39 Schachman, H. K. 1959. Ultracentrifugation in Biochemistry. Academic Press. New York . Pp. 75– 85. 40 Longsworth, L. G. 1952. Diffusion measurements, at 1°, of aqueous solutions of amino acids, peptides and sugars. J. Am. Chem. Soc. 74: 4155– 4159. 41 Spackman, D. H., W. H. Stein & S. Moore. 1958. Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem. 30: 1190– 1206. 42 Moore, S. 1963. On the determination of cystine as cysteic acid. J. Biol. Chem. 238: 235– 237. 43 Sanger, F. 1945. The free amino groups of insulin. Biochem. J. 39: 507– 515. 44 Rigas, D. A. 1958. Kinetics of isotope incorporation into the desoxyribonucleic acid (DNA) of tissues: Life span and generation time of cells. Bull. Math. Biophys. 20: 33– 70. 45 Rigas, D. A. 1959. Dynamics of cell proliferation and isotope incorporation into deoxyribonucleic acid. In The Kinetics of Cellular Proliferation. F. Stohlman, Ed. Grune & Stratton. New York . Pp. 408– 430. 46 Rigas, D. A. 1962. Theoretical considerations of cellular proliferation as studied by celllabeling processes. Ann. N. Y. Acad. Sci. 96: 1008– 1028. 47 Svedberg, THE & K. O. Pedersen. 1940. The ultracentrifuge. Oxford Univ. Press. London and New York . (Johnson Reprint Corporation, New York.) P. 5. 48 Bourdillon, J. 1949. Crystalline protein from commercial beans (Phaseolus vulgaris). J. Biol. Chem. 180: 553– 556. 49 Bourdillon, J. 1951. A crystalline bean seed protein in combination with phytic acid. J. Biol Chem. 189: 65– 72. 50 Punnett, TH., H. H. Punnett & B. N. Kaufmann. 1962. Preparation of a crude human leukocyte growth factor from Phaseolis vulgaris. Lancet. 1: 1359– 1360. 51 Renkonen, K. O. & K. Harjanne. 1961. The hemagglutinins and their inhibitors in the seeds of vicia cracca. Ann. Med. Exp. et Biol. Fenniae (Helsinki). 39: 313– 315. 52 Jaffé, W. G. 1949. Toxicity of raw kidney-beans. Experientia. 5: 81. 53 Jaffé, W. G. 1950. Estudio sobre la inhibición del crecimiento de ratas causada por algunas de leguminosas. Acta Cient. Venezolana. 1: 62– 64. 54 Jaffé, W. G. & K. Gaede. 1959. Purification of a toxic phytohaemagglutinin from black beans (Phaseolus vulgaris). Nature (London). 183: 1329– 1330. 55 Piekarski, L. 1957. Badania nad Fazyna. Osobne Odbicie Z “Dissertationes Pharmaceutical.”9: 255– 263. 56 Piekarski, L. 1957. Aminokwasy wystepujace w toksycznych bialkach roslin straczkowych. Odbitka z czasopisma Acta Polonial Pharmaceutica. 1: 47– 49. Citing Literature Volume113, Issue2Leukopoiesis in Health and DiseaseFebruary 1964Pages 800-818 ReferencesRelatedInformation