Identification and Characterization of CDS2, a Mammalian Homolog of theDrosophilaCDP-diacylglycerol Synthase Gene
1999; Elsevier BV; Volume: 55; Issue: 1 Linguagem: Inglês
10.1006/geno.1998.5610
ISSN1089-8646
AutoresManuela Volta, Alessandro Bulfone, Claudio Gattuso, Elena Rossi, Margherita Mariani, G. Giacomo Consalez, Orsetta Zuffardi, Andrea Ballabio, Sandro Banfi, Brunella Franco,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoThe general strategies of phototransduction in vertebrates and invertebrates share many similarities, but differ significantly in their underlying molecular machinery. TheCDSgene encodes theCDP-diacylglycerol synthase(CDS) enzyme and is required for phototransduction inDrosophila.Using a bioinformatic approach, we have identified two novel transcripts (CDS1andCDS2) highly homologous to theDrosophila CDSgene. We isolated and sequenced theCDS2full-length cDNA and mapped the two genes to human chromosomes 20p13 (CDS2) and 4q21.1 (CDS1). Sequence analysis revealed that both genes are highly homologous to theDrosophilaprotein (64.4 and 58.6% identity at the protein level betweenCDSandCDS2and betweenCDSandCDS1,respectively). The mouse homologs for both genes were isolated and used in RNAin situhybridization studies on adult and embryonic mouse tissue sections. These studies showed thatCds2is highly expressed in the differentiating neuroblasts of the neural retina and in the central nervous system during embryonic development, while it was not detected in adult retina.Cds1,on the other hand, shows a high level of expression in the photoreceptor layer of adult retina, which strongly suggests a role forCds1in phototransduction. Knowledge of the expression pattern of these genes in mammals may shed light on the evolution of vision mechanisms and help in the evaluation of candidate genes for human retinopathies.
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