Lysine as a muscle cation in potassium deficiency
1954; Elsevier BV; Volume: 52; Issue: 1 Linguagem: Inglês
10.1016/0003-9861(54)90118-6
ISSN1096-0384
AutoresRobert E. Eckel, Charles E. Pope, James E Norris,
Tópico(s)Biotin and Related Studies
Resumo1.1. Left ventricular slices of male Sprague-Dawley rats were incubated with a fixed concentration of 0.5 μCi/ml 3H-lysine and several concentrations of unlabelled lysine ranging from 0.2 to 5.0 mM in control and prostacylin-treated experiments. The time of incubation ranged from 0.5 to 90 min.2.2. Left ventricular slices were cut to have an optimal thickness of 0.47 ± 0.09 mm.3.3. Lysine was taken up against a concentration gradient. Saturation was reached at 0.5 mM and steady state accumulation of lysine was attained within 60 min.4.4. Prostacyclin in concentrations ranging from 1.2 × 10−8 to 4.8 × 10−8 M inhibited lysine transport in left ventricular slices significantly (P < 0.01).
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