Improved Recovery of Rabies Virus from Cloned cDNA Using a Vaccinia Virus‐Free Reverse Genetics System
2003; Wiley; Volume: 47; Issue: 8 Linguagem: Inglês
10.1111/j.1348-0421.2003.tb03424.x
ISSN1348-0421
AutoresNaoto Ito, Mutsuyo Takayama‐Ito, Kentaro Yamada, Junji Hosokawa, Makoto Sugiyama, Nobuyuki Minamoto,
Tópico(s)Virus-based gene therapy research
ResumoAbstract To improve efficiency of recovery of rabies virus from cloned cDNA, we established a BHK cell clone that stably expresses T7 RNA polymerase, which we named BHK/T7–9. We also constructed new helper plasmids for expression of nucleoprotein and RNA polymerase of the RC‐HL strain using the pTM1 plasmid vector, which makes the T7 RNA polymerase‐transcripts from the plasmid cap‐independent for translation. After co‐transfection of these helper plasmids and the previously constructed full‐length genome plasmid of the RC‐HL strain to BHK/T7–9 cells, recombinant rabies virus was efficiently recovered from the cloned cDNA.
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