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Molecular typing of HLA class II antigens in a São Paulo population

1998; Brazilian Society of Genetics; Volume: 21; Issue: 3 Linguagem: Inglês

10.1590/s1415-47571998000300001

1678-4685

Anna Carla Goldberg, J.M. Chiarella, Maria Lúcia Carnevale Marin, César Rosales, Dalma Maria Banic, Marco Aurelio Tuena de Oliveira, H. Rodrigues, C. Viggiani, Jorge Kalil,

T-cell and B-cell Immunology

In the present paper we show data obtained from a normal population with a racially mixed profile typical of the city of São Paulo, State of São Paulo. Data were generated with polymerase chain reaction using sequence specific primers (PCR-SSP) for HLA-DRB and polymerase chain reaction followed by hybridization with sequence specific oligonucleotide probes (PCR-SSO) for HLA-DQA1 and HLA-DQB1 loci. HLA-DRB, DQA1, DQB1 and haplotype frequencies as well as common linkage disequilibria were found. This population was also shown to be in genetic equilibrium according to the Hardy-Weinberg law. HLA-DR typing of a normal sample from the city of Porto Velho, State of Rondonia, highlighted the importance of different sets of HLA profiles found in other regions of the country. This database provides essential information for screening studies of disease associations, forensic analyses and transplants.