Produção Nacional
Revisado por pares
Regulatory T cells in the actinic cheilitis
2014; Wiley; Volume: 43; Issue: 10 Linguagem: Inglês
10.1111/jop.12207
ISSN1600-0714
AutoresThaís Helena Gasparoto, Tatiana Salles de Souza Malaspina, José Humberto Damante, Edgard Franco de Mello, Maura Rosane Valério Ikoma, Gustavo Pompermaier Garlet, Maria Renata Sales Nogueira Costa, Karen A. Cavassani, João S. Silva, Ana Paula Campanelli,
Tópico(s)Immunotherapy and Immune Responses
ResumoBackground Actinic cheilitis ( AC ) is an oral potentially malignant lesion which is the counterpart of actinic keratosis of the skin and has potential to develop into squamous cell carcinoma. Regulatory T cells (Tregs) have a critical role in modulating the antitumor immune responses. The presence of regulatory T cells in potentially malignant lesions has not been described. We chose investigate the involvement of regulatory T cells in potentially malignant lesions. Methods The frequency, phenotype, and activity of CD 4+ CD 25+ T cells isolated from blood and lesion of AC patients were analyzed by flow cytometry. Cytokines were quantified by ELISA . Data were compared with samples from healthy subjects. Results The frequency and suppressor activity of circulating CD 4+ CD 25+ T cells was similar in AC patients and control subjects. However, the frequencies of IL ‐10‐positive Tregs were higher in AC patients, and these cells inhibited interferon‐gamma ( IFN ‐γ) and increased interleukin ( IL )‐10 productions in co‐cultures. Furthermore, CD 4+ CD 25+ T cells accumulate in AC lesions. Lesions‐derived regulatory T cells suppressed lymphocyte proliferation and pro‐inflammatory cytokine production. Moreover, high levels of IL ‐10 and transforming growth factor‐β ( TGF ‐β), and low IFN ‐γ were detected in the potentially malignant lesions. Conclusion Therefore, our data show that Tregs accumulate in AC lesions, and these cells could be suppressing immune responses in a potentially malignant microenvironment.
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