Enhancement Effects of High-Energy Neon Particles on the Viral Transformation of Mouse C3H1OT1/2 Cells in Vitro
1980; Radiation Research Society; Volume: 81; Issue: 2 Linguagem: Inglês
10.2307/3575397
ISSN1938-5404
AutoresT. C. Yang, Cornelius A. Tobias, Eleanor A. Blakely, Laurie M. Craise, Ian S. Madfes, Carl F. Perez, Jerry Howard,
Tópico(s)DNA Repair Mechanisms
ResumoThe enhancement effects of X rays and high-LET particles on the viral transformation of cultured mammalian cells were studied. Confluent mouse C3H1OT1/2 cells, x-irradiated immediately after infection with Simian virus (SV40), showed a higher frequency of transformation than cells infected only with the virus. The enhancement of viral transformation appeared to be dose-dependent and more than additive. The number of transformations per cell increased with dose and reached a maximum at about 300 rad. The frequency of transformation per survivor was enhanced linearly with the square of the x-ray dose. Compared to X rays, neon particles with a residual range of 12.7 g/cm/sup 2/ in water were much more efficient in enhancing the viral transformation of cells. The number of transformants per plated cell reached a peak around 200 rad. The RBE for enhancing the frequency of transformation per survivor appeared to vary with dose, being higher at lower dose. For the 50% survival level dose of neon ions, the RBE was found to be about 2.28 compared to about 1.84 for the 10% survival level dose. A decrease in the enhancement effect was found as the interval between the irradiation and viral infection was extended. This suggests that themore » cells in the G/sub 1/ phase can repair the radiation-induced lesions that are responsible for the observed increase of transformation. The recovery rate for cells irradiated with neon ions, however, was much slower than that for cells irradiated with X rays.« less
Referência(s)