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Reversible bilayer junction of lipid monolayers: free mono-bi-monolayer contact

1986; Elsevier BV; Volume: 861; Linguagem: Inglês

10.1016/0005-2736(86)90435-9

1879-2642

Karl Heinz Dambacher, Peter Fromherz,

Force Microscopy Techniques and Applications

A stable lipid bilayer is formed from two lipid monolayers at the air/water interface such that the three-phase contact monolayer-bilayer-monolayer is free from any support ('MBM technique'). The bilayers are made from cardiolipin and glycerin monooleate spread from a volatile solvent. Joining and disjoining of the monolayers to a quasi-solvent-free bilayer is reversible. The process is characterized by video and by recording capacitance. The new method provides the basis for a formation of bilayers in equilibrium with monolayers on two Langmuir troughs in a controlled state.