Portal de Periódicos da CAPES

Massively parallel delivery of large cargo into mammalian cells with light pulses

2015; Nature Portfolio; Volume: 12; Issue: 5 Linguagem: Inglês

10.1038/nmeth.3357

1548-7105

Yi-Chien Wu, Ting‐Hsiang Wu, Daniel L. Clemens, Bai‐Yu Lee, Ximiao Wen, Marcus A. Horwitz, Michael A. Teitell, Pei‐Yu Chiou,

Cancer Research and Treatments

The biophotonic laser-assisted surgery tool (BLAST) enables the delivery of nearly any cargo type, including micrometer-sized live bacteria, into a wide variety of cell types with high efficiency, throughput and cell viability. We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle of Francisella novicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.