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Shrimp thioredoxin is a potent antioxidant protein

2008; Elsevier BV; Volume: 148; Issue: 1 Linguagem: Inglês

10.1016/j.cbpc.2008.03.013

1878-1659

Emmanuel Aispuro‐Hernández, Karina D. García‐Orozco, Adriana Muhlia‐Almazán, Carmen Lizette Del‐Toro‐Sánchez, Rosario Maribel Robles-Sánchez, Jesús Hernández, Gustavo A. González‐Aguilar, Glória Yépiz-Plascencia, Rogerio R. Sotelo‐Mundo,

Genetics, Aging, and Longevity in Model Organisms

Thioredoxin (TRX) is a main component of the redox homeostasis machinery in the cell and it is required for ribonucleotide reductase function among others. In invertebrates, the redox balance is compromised during disease and changes in the physiological state and it is one of the components of the innate immune response. In this work, the shrimp (Litopenaeus vannamei) LvTRX cDNA was sequenced, cloned and over-expressed in bacteria to further characterize the function of the recombinant protein. LvTRX was able to reduce insulin disulfides and it was a better antioxidant compared to reduced glutathione and ascorbic acid, by means of the Trolox Equivalent Antioxidant Capacity (TEAC) assay. Interestingly, LvTRX contains aside of the canonical active site CXXC disulfide motif, one Cys (C73) residue in the interface of a putative dimer previously reported for human TRX. Using qRT-PCR, we found that shrimp LvTRX is mainly expressed in gills and pleopods; the variation of LvTRX mRNA upon hypoxia and re-oxygenation is not statistically significant. LvTRX stands as an important antioxidant that must be considered in future physiological and immune challenges studies.