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Influência do potencial hídrico induzido por polietilenoglicol in vitro na morfologia do trigo

2014; UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; Volume: 9; Issue: 3 Linguagem: Inglês

10.5039/agraria.v9i3a3710

ISSN

1981-1160

Autores

Felipe Favoretto Furlan, Kléber Saatkamp, Maik Cleiton Wiest, Marise Fonseca dos Santos, Eliane Cristina Gruszka Vendruscolo,

Tópico(s)

Soil Management and Crop Yield

Resumo

Plant´s growing depends on several environmental factors that determine their productivity. Drought is a limiting factor for crop production. The aim of this study was the establishment of a protocol for evaluating in vitro, the water stress effect on wheat seedlings. Treatments consisted of simulating the following water potentials (-0.1; -0.2; -0.3 and -0.4 MPa) using PEG - 6000 and the control. The experimental design was completely randomized with five treatments and five repetitions. It was evaluated morphological and physiological parameters such as: relative water content (RWC), membrane stability index (MEI) and the roots morphology by microscopy. As results, we observed that -0.3 MPa (PEG 6000 151.41 g L-1) osmotic potential and the 5 days period provided similar effects of water shortage as that one occurring at green house conditions, which is suitable for simulating this abiotic stress at laboratory conditions.

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