Hemolysate-filled polyethyleneimine and polyurea microcapsules as potential red blood cell substitutes: effect of aqueous monomer type on properties of the prepared microcapsules
2004; Elsevier BV; Volume: 278; Issue: 1 Linguagem: Inglês
10.1016/j.ijpharm.2004.01.031
ISSN1873-3476
Autores Tópico(s)Pancreatic function and diabetes
ResumoIn this paper, we describe the synthesis and characterization of rabbit hemolysate-filled polyethyleneimine (PEI)- or polyurea (PU)-type artificial red blood cells (ARBCs) with different membrane compositions. These microcapsules were prepared by making use of the interfacial polymerization (IP) reaction between the water-soluble amine monomers (triethylamine (TEA), ethylene glycol-bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGATA), diethylenetriamine (DETA), tetramethyl diaminomethane (TMDAM), piperazine hexahydrate (PPHH), l-lysine monohydrochloride (LLMH) or PEI) and 2,4-toluylene diisocyanate (TDI) as an oil-soluble shell monomer. The resultant microcapsules were spherical and with mean diameters of 8.71-63.33 microm. Microcapsules having sulfonic acid groups on their surfaces were prepared by using a combination of the functional amines (DETA, LLMH or PEI) and 4,4'-diaminostilbene-2,2'-disulfonic acid (DASSA). Oxygen-binding abilities of the ARBCs were measured by a Clark-type oxygen electrode. The obtained results revealed that the highest oxygen-binding abilities were obtained with the PU-ARBCs prepared with DETA alone or in combination with EGATA. Unfortunately, these microcapsules exhibited large diameters and wider size distribution curves (span values (S) = 1.3, 1.7, geometric standard deviation sigma(g) = 1.85, 2.18, respectively). However, the novel ARBCs (sulfonated PU-PEI graft copolymer membrane microcapsules (SPU/PEI-ARBCs)) prepared had good oxygen affinity, the smallest mean diameter (d = 8.71 microm) and the best distribution (S = 0.9, sigma(g) = 1.48) and a flow behavior identical to rabbit RBCs. Therefore, these unique microcapsules can be recommended for scale-up considerations as a promising blood substitute.
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