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Dietary Nucleotides Increase the Proportion of a TCRγδ + Subset of Intraepithelial Lymphocytes (IEL) and IL-7 Production by Intestinal Epithelial Cells (IEC); Implications for Modification of Cellular and Molecular Cross-talk between IEL and IEC by Dietary Nucleotides

2000; Oxford University Press; Volume: 64; Issue: 7 Linguagem: Inglês

10.1271/bbb.64.1459

1347-6947

Shinya Nagafuchi, Mamoru Totsuka, Satoshi HACHIMURA, Masao GOTO, Takeshi Takahashi, Takaji Yajima, Tamotsu Kuwata, Shuichi Kaminogawa,

Helicobacter pylori-related gastroenterology studies

We have investigated the effects of dietary nucleotides on intraepithelial lymphocytes (IEL) and intestinal epithelial cells (IEC) in weanling mice. The proportion of T-cell receptor (TCR) γδ+ IEL in BALB/c mice fed a diet supplemented with nucleotides (NT(+) diet) was significantly higher than that in mice fed the nucleotide-free diet, while the proportion of TCRαβ+ IEL in NT(+) diet-fed mice was significantly decreased. The change of the TCRαβ+/TCRγδ+ ratio was mainly observed in a CD8αα+ subset of IEL. IEC from NT(+) diet-fed mice produced a higher level of IL-7, which is important in the development of TCRγδ+ IEL, than those from control diet-fed mice. The expression levels of IL-7 and IL-2 receptors on IEL were not different between the two dietary groups. Our findings suggest that the increased population of a TCRγδ+ IEL subset by feeding nucleotides may be caused by the increased production of IL-7 by IEC.