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Inhibition of Bladder Tumor Growth by the Green Tea Derivative Epigallocatechin-3-Gallate

2003; Lippincott Williams & Wilkins; Volume: 170; Issue: 3 Linguagem: Inglês

10.1097/01.ju.0000081278.64511.96

1527-3792

J. KARL KEMBERLING, James A. Hampton, Rick W. Keck, Michael A. Gomez, Steven H. Selman,

Phytoestrogen effects and research

No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles1 Sep 2003Inhibition of Bladder Tumor Growth by the Green Tea Derivative Epigallocatechin-3-Gallate J. KARL KEMBERLING, JAMES A. HAMPTON, RICK W. KECK, MICHAEL A. GOMEZ, and STEVEN H. SELMAN J. KARL KEMBERLINGJ. KARL KEMBERLING , JAMES A. HAMPTONJAMES A. HAMPTON , RICK W. KECKRICK W. KECK , MICHAEL A. GOMEZMICHAEL A. GOMEZ , and STEVEN H. SELMANSTEVEN H. SELMAN View All Author Informationhttps://doi.org/10.1097/01.ju.0000081278.64511.96AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We evaluated the green tea derivative epigallocatechin-3-gallate (EGCG) as an intravesical agent for the prevention of transitional cell tumor implantation. Materials and Methods: In vitro studies were performed in the AY-27 rat transitional cell cancer and the L1210 mouse leukemia cell lines. Cells were exposed to increasing concentrations of EGCG for 30 minutes to 48 hours. Surviving cell colonies were then determined. A DNA ladder assay was performed in the 2 cell lines. Fisher 344 rats were used for in vivo studies with an intravesical tumor implantation model. Group 1 (12 rats) served as a control (tumor implantation and medium wash only). In group 2 (28 rats) 200 μM EGCG were instilled intravesically 30 minutes after tumor implantation. Rats were sacrificed 3 weeks following treatment. Gross and histological analyses were then performed on the bladders. Results: At 6.0 × 104 cells per 100 mm dish a time dose dependent response was observed. After 2 hours of treatment with EGCG 100% cell lethality of the AY-27 cell line occurred at concentrations greater than 100 μM. Strong banding on the DNA ladder assay was seen with the L1210 mouse leukemia cell line. Only weak banding patterns were found in the AY-27 cell line treated with EGCG (100 and 200 μM) for 24 hours. All 12 controls were successfully implanted with tumors. In group 2 (EGCG instillation) 18 of the 28 animals (64%) were free of tumor (Fisher's exact test p = 0.001). Conclusions: The clonal assays showed a time dose related response to EGCG. Intravesical instillation of EGCG inhibits the growth of AY-27 rat transitional cells implanted in this model. References 1 : The clinical epidemiology of superficial bladder cancer. Dutch South-East Cooperative Urological Group. Br J Cancer1993; 67: 806. Google Scholar 2 : The place of intravesical chemotherapy as defined by results of prospective randomized studies (substances and treatment schemes). Prog Clin Biol Res1992; 378: 43. Google Scholar 3 : Susceptibility of urothelium to neoplastic cellular implantation. Urology1975; 5: 824. Crossref, Medline, Google Scholar 4 : Prevention of human bladder tumor cell implantation in an in vitro assay. J Urol1987; 137: 777. Google Scholar 5 : Green tea: healing tonic. Am J Natur Med1998; 5: 28. 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Link, Google Scholar From the Departments of Urology (JKK, RWK, MAG, SHS) and Pathology (JAH), Medical College of Ohio, Toledo, Ohio© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySelman S and Keck R (2011) A Comparative Study of the Inhibiting Effects of Mitomycin C and Polyphenolic Catechins on Tumor Cell Implantation/Growth in a Rat Bladder Tumor ModelJournal of Urology, VOL. 186, NO. 2, (702-706), Online publication date: 1-Aug-2011.Berrahmoune S, Bezdetnaya L, Leroux A, Guillemin F and D'Hallewin M (2009) Preventing Bladder Tumor Implantation With Photodynamic Therapy in a Rat Model Mimicking Post-Fluorescence Guided Transurethral ResectionJournal of Urology, VOL. 181, NO. 3, (1381-1386), Online publication date: 1-Mar-2009. Volume 170Issue 3September 2003Page: 773-776 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordsepicatechincarcinoma, transitional cellteabladderrats, inbred F344Metrics Author Information J. KARL KEMBERLING More articles by this author JAMES A. HAMPTON More articles by this author RICK W. KECK More articles by this author MICHAEL A. GOMEZ More articles by this author STEVEN H. SELMAN Financial interest and/or other relationship with QLT, Inc. More articles by this author Expand All Advertisement PDF downloadLoading ...